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  Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells

Slováková, J., Sikora, M., Arslan, F. N., Caballero-Mancebo, S., Krens, S. F. G., Kaufmann, W. A., et al. (2022). Tension-dependent stabilization of E-cadherin limits cell-cell contact expansion in zebrafish germ-layer progenitor cells. Proceedings of the National Academy of Sciences of the United States of America, 119(8): e2122030119. doi:10.1073/pnas.2122030119.

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 Creators:
Slováková, Jana1, 2, Author
Sikora, Mateusz3, Author              
Arslan, Feyza Nur, Author
Caballero-Mancebo, Silvia, Author
Krens, S. F. Gabriel, Author
Kaufmann, Walter A., Author
Merrin, Jack, Author
Heisenberg, Carl-Philipp, Author
Affiliations:
1Institute of Science and Technology Austria, Klosterneuburg, Austria, ou_persistent22              
2Department of Theoretical Biophysics, Institute of Molecular Biotechnology, Vienna, Austria, ou_persistent22              
3Department of Theoretical Biophysics, Max Planck Institute of Biophysics, Max Planck Society, ou_2068292              

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Free keywords: cell adhesion, cell–cell contact formation, mechanosensing
 Abstract: Tension of the actomyosin cell cortex plays a key role in determining cell-cell contact growth and size. The level of cortical tension outside of the cell-cell contact, when pulling at the contact edge, scales with the total size to which a cell-cell contact can grow [J.-L. Maître et al., Science 338, 253-256 (2012)]. Here, we show in zebrafish primary germ-layer progenitor cells that this monotonic relationship only applies to a narrow range of cortical tension increase and that above a critical threshold, contact size inversely scales with cortical tension. This switch from cortical tension increasing to decreasing progenitor cell-cell contact size is caused by cortical tension promoting E-cadherin anchoring to the actomyosin cytoskeleton, thereby increasing clustering and stability of E-cadherin at the contact. After tension-mediated E-cadherin stabilization at the contact exceeds a critical threshold level, the rate by which the contact expands in response to pulling forces from the cortex sharply drops, leading to smaller contacts at physiologically relevant timescales of contact formation. Thus, the activity of cortical tension in expanding cell-cell contact size is limited by tension-stabilizing E-cadherin-actin complexes at the contact.

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Language(s): eng - English
 Dates: 2021-12-132021-12-292022-02-142022-02-22
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.2122030119
BibTex Citekey: slovakova_tension-dependent_2022
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Affiliations:
Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 119 (8) Sequence Number: e2122030119 Start / End Page: - Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230