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Abstract:
Using cryo-microscopy at liquid-helium temperature we have determined the projection structure of the surface layer of the archaebacterium Sulfolobus shibatae embedded in aurothioglucose to a resolution of 0.85 nm. Image analysis was performed in real space and incorporated a classification of unit cells, thus taking into account the frequent occurrence of twin boundaries. In an attempt to identify resolution-limiting factors we have performed a distortion analysis quantifying the main distortion parameters.
