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  Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads

Belicova, L., Repnik, U., Delpierre, J., Gralinska, E., Seifert, S., Valenzuela, J. I., et al. (2021). Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads. The Journal of Cell Biology: JCB, 220(10): e202103003. doi:10.1083/jcb.202103003.

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JCB_Belicova et al_2021.pdf (Publisher version), 9MB
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© 2021 Belicova et al

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 Creators:
Belicova , Lenka , Author
Repnik , Urska , Author
Delpierre , Julien , Author
Gralinska, Elzbieta1, Author              
Seifert , Sarah , Author
Valenzuela , José Ignacio , Author
Morales-Navarrete , Hernán Andrés , Author
Franke , Christian , Author
Räägel , Helin , Author
Shcherbinina , Evgeniya , Author
Prikazchikova , Tatiana , Author
Koteliansky , Victor , Author
Vingron, Martin1, Author              
Kalaidzidis , Yannis L. , Author
Zatsepin , Timofei , Author
Zerial , Marino , Author
Affiliations:
1Transcriptional Regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              

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 Abstract: Lumen morphogenesis results from the interplay between molecular pathways and mechanical forces. In several organs, epithelial cells share their apical surfaces to form a tubular lumen. In the liver, however, hepatocytes share the apical surface only between adjacent cells and form narrow lumina that grow anisotropically, generating a 3D network of bile canaliculi (BC). Here, by studying lumenogenesis in differentiating mouse hepatoblasts in vitro, we discovered that adjacent hepatocytes assemble a pattern of specific extensions of the apical membrane traversing the lumen and ensuring its anisotropic expansion. These previously unrecognized structures form a pattern, reminiscent of the bulkheads of boats, also present in the developing and adult liver. Silencing of Rab35 resulted in loss of apical bulkheads and lumen anisotropy, leading to cyst formation. Strikingly, we could reengineer hepatocyte polarity in embryonic liver tissue, converting BC into epithelial tubes. Our results suggest that apical bulkheads are cell-intrinsic anisotropic mechanical elements that determine the elongation of BC during liver tissue morphogenesis.

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Language(s): eng - English
 Dates: 2021-07-082021-07-30
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1083/jcb.202103003
 Degree: -

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Title: The Journal of Cell Biology : JCB
  Other : J. Cell Biol.
Source Genre: Journal
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Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 220 (10) Sequence Number: e202103003 Start / End Page: - Identifier: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024