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  Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads

Belicova, L., Repnik, U., Delpierre, J., Gralinska, E., Seifert, S., Valenzuela, J. I., et al. (2021). Anisotropic expansion of hepatocyte lumina enforced by apical bulkheads. The Journal of Cell Biology: JCB, 220(10): e202103003. doi:10.1083/jcb.202103003.

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JCB_Belicova et al_2021.pdf (Verlagsversion), 9MB
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© 2021 Belicova et al

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 Urheber:
Belicova , Lenka , Autor
Repnik , Urska , Autor
Delpierre , Julien , Autor
Gralinska, Elzbieta1, Autor           
Seifert , Sarah , Autor
Valenzuela , José Ignacio , Autor
Morales-Navarrete , Hernán Andrés , Autor
Franke , Christian , Autor
Räägel , Helin , Autor
Shcherbinina , Evgeniya , Autor
Prikazchikova , Tatiana , Autor
Koteliansky , Victor , Autor
Vingron, Martin1, Autor           
Kalaidzidis , Yannis L. , Autor
Zatsepin , Timofei , Autor
Zerial , Marino , Autor
Affiliations:
1Transcriptional Regulation (Martin Vingron), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479639              

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 Zusammenfassung: Lumen morphogenesis results from the interplay between molecular pathways and mechanical forces. In several organs,
epithelial cells share their apical surfaces to form a tubular lumen. In the liver, however, hepatocytes share the apical surface only between adjacent cells and form narrow lumina that grow anisotropically, generating a 3D network of bile canaliculi (BC). Here, by studying lumenogenesis in differentiating mouse hepatoblasts in vitro, we discovered that adjacent hepatocytes assemble a pattern of specific extensions of the apical membrane traversing the lumen and ensuring its anisotropic expansion. These previously unrecognized structures form a pattern, reminiscent of the bulkheads of boats, also present in the developing and adult liver. Silencing of Rab35 resulted in loss of apical bulkheads and lumen anisotropy, leading to cyst formation. Strikingly, we could reengineer hepatocyte polarity in embryonic liver tissue, converting BC into epithelial tubes.
Our results suggest that apical bulkheads are cell-intrinsic anisotropic mechanical elements that determine the elongation of BC during liver tissue morphogenesis.

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Sprache(n): eng - English
 Datum: 2021-07-082021-07-30
 Publikationsstatus: Online veröffentlicht
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 Identifikatoren: DOI: 10.1083/jcb.202103003
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Titel: The Journal of Cell Biology : JCB
  Andere : J. Cell Biol.
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: New York, NY : Rockefeller Institute Press
Seiten: - Band / Heft: 220 (10) Artikelnummer: e202103003 Start- / Endseite: - Identifikator: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024