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  Promoter Activation in Delta hfq Mutants as an Efficient Tool for Specialized Metabolite Production Enabling Direct Bioactivity Testing

Bode, E., Heinrich, A. K., Hirschmann, M., Abebew, D., Shi, Y.-N., Tien Vo, D., et al. (2019). Promoter Activation in Delta hfq Mutants as an Efficient Tool for Specialized Metabolite Production Enabling Direct Bioactivity Testing. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 58(52), 18957-18963. doi:10.1002/anie.201910563.

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 Creators:
Bode, Edna1, Author           
Heinrich, Antje K.2, Author
Hirschmann, Merle2, Author
Abebew, Desalegne2, Author
Shi, Yan-Ni1, Author           
Tien Vo, Duy2, Author
Wesche, Frank2, Author
Shi, Yi-Ming1, Author           
Gruen, Peter2, Author
Simonyi, Svenja2, Author
Keller, Nadine2, Author
Engel, Yvonne2, Author
Wenski, Sebastian2, Author
Bennet, Reuel2, Author
Beyer, Sophie2, Author
Bischoff, Iris2, Author
Buaya, Anthony2, Author
Brandt, Sophie2, Author
Cakmak, Ibrahim2, Author
Cimen, Harun2, Author
Eckstein, Simone2, AuthorFrank, Denia2, AuthorFuerst, Robert2, AuthorGand, Martin2, AuthorGeisslinger, Gerd2, AuthorHazir, Selcuk2, AuthorHenke, Marina2, AuthorHeermann, Ralf2, AuthorLecaudey, Virginie2, AuthorSchaefer, Wilhelm2, AuthorSchiffmann, Susanne2, AuthorSchueffler, Anja2, AuthorSchwenk, Rebecca2, AuthorSkaljac, Marisa2, AuthorThines, Eckhard2, AuthorThines, Marco2, AuthorUlshoefer, Thomas2, AuthorVilcinskas, Andreas1, Author           Wichelhaus, Thomas A.2, AuthorBode, Helge B.3, Author            more..
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1External Organizations, ou_persistent22              
2external, ou_persistent22              
3Goethe-Universität Frankfurt am Main, External Organizations, ou_421891              

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 Abstract: Natural products (NPs) from microorganisms have been important sources for discovering new therapeutic and chemical entities. While their corresponding biosynthetic gene clusters (BGCs) can be easily identified by gene-sequencesimilarity-based bioinformatics strategies, the actual access to these NPs for structure elucidation and bioactivity testing remains difficult. Deletion of the gene encoding the RNA chaperone, Hfq, results in strains losing the production of most NPs. By exchanging the native promoter of a desired BGC against an inducible promoter in Dhfq mutants, almost exclusive production of the corresponding NP from the targeted BGC in Photorhabdus, Xenorhabdus and Pseudomonas was observed including the production of several new NPs derived from previously uncharacterized non-ribosomal peptide synthetases (NRPS). This easyPACId approach (easy Promoter Activated Compound Identification) facilitates NP identification due to low interference from other NPs. Moreover, it allows direct bioactivity testing of supernatants containing secreted NPs, without laborious purification.

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 Dates: 2019
 Publication Status: Issued
 Pages: -
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 Rev. Type: -
 Identifiers: ISI: 000502568200001
DOI: 10.1002/anie.201910563
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Title: ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Source Genre: Journal
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Pages: - Volume / Issue: 58 (52) Sequence Number: - Start / End Page: 18957 - 18963 Identifier: ISSN: 1433-7851