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  Nonacetogenic Growth of the Acetogen Acetobacterium woodii on 1,2-Propanediol

Schuchmann, K., Schmidt, S., Lopez, A. M., Kaberline, C., Kuhns, M., Lorenzen, W., et al. (2015). Nonacetogenic Growth of the Acetogen Acetobacterium woodii on 1,2-Propanediol. JOURNAL OF BACTERIOLOGY, 197(2), 382-391. doi:10.1128/JB.02383-14.

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 Creators:
Schuchmann, Kai1, Author
Schmidt, Silke1, Author
Lopez, Antonio Martinez1, Author
Kaberline, Christina1, Author
Kuhns, Martin1, Author
Lorenzen, Wolfram1, Author
Bode, Helge B.2, Author           
Joos, Friederike, Author           
Mueller, Volker1, Author
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1external, ou_persistent22              
2Goethe-Universität Frankfurt am Main, External Organizations, ou_421891              

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 Abstract: Acetogenic bacteria can grow by the oxidation of various substrates coupled to the reduction of CO2 in the Wood-Ljungdahl pathway. Here, we show that growth of the acetogen Acetobacterium woodii on 1,2-propanediol (1,2-PD) as the sole carbon and energy source is independent of acetogenesis. Enzymatic measurements and metabolite analysis revealed that 1,2-PD is dehydrated to propionaldehyde, which is further oxidized to propionyl coenzyme A (propionyl-CoA) with concomitant reduction of NAD. NADH is reoxidized by reducing propionaldehyde to propanol. The potential gene cluster coding for the responsible enzymes includes genes coding for shell proteins of bacterial microcompartments. Electron microscopy revealed the presence of microcompartments as well as storage granules in cells grown on 1,2-PD. Gene clusters coding for the 1,2-PD pathway can be found in other acetogens as well, but the distribution shows no relation to the phylogeny of the organisms.

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 Dates: 2015
 Publication Status: Issued
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 Identifiers: ISI: 000347676200015
DOI: 10.1128/JB.02383-14
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Title: JOURNAL OF BACTERIOLOGY
Source Genre: Journal
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Pages: - Volume / Issue: 197 (2) Sequence Number: - Start / End Page: 382 - 391 Identifier: ISSN: 0021-9193