English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Genetic analysis of xenocoumacin antibiotic production in the mutualistic bacterium Xenorhabdus nematophila

Park, D., Ciezki, K., van der Hoeven, R., Singh, S., Reimer, D., Bode, H. B., et al. (2009). Genetic analysis of xenocoumacin antibiotic production in the mutualistic bacterium Xenorhabdus nematophila. MOLECULAR MICROBIOLOGY, 73(5), 938-949. doi:10.1111/j.1365-2958.2009.06817.x.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Park, Dongjin1, Author
Ciezki, Kristin1, Author
van der Hoeven, Ransome1, Author
Singh, Swati1, Author
Reimer, Daniela1, Author
Bode, Helge B.2, Author           
Forst, Steven1, Author
Affiliations:
1external, ou_persistent22              
2Goethe-Universität Frankfurt am Main, External Organizations, ou_421891              

Content

show
hide
Free keywords: -
 Abstract: P>Xenocoumacin 1 (Xcn1) and xenocoumacin 2 (Xcn2) are the major antimicrobial compounds produced by Xenorhabdus nematophila. To study the role of Xcn1 and Xcn2 in the life cycle of X. nematophila the 14 gene cluster (xcnA-N) required for their synthesis was identified. Overlap RT-PCR analysis identified six major xcn transcripts. Individual inactivation of the non-ribosomal peptide synthetase genes, xcnA and xcnK, and polyketide synthetase genes, xcnF, xcnH and xcnL, eliminated Xcn1 production. Xcn1 levels and expression of xcnA-L were increased in an ompR strain while Xcn2 levels and xcnMN expression were reduced. Xcn1 production was also increased in a strain lacking acetyl-phosphate that can donate phosphate groups to OmpR. Together these findings suggest that OmpR-phosphate negatively regulates xcnA-L gene expression while positively regulating xcnMN expression. HPLC-MS analysis revealed that Xcn1 was produced first and was subsequently converted to Xcn2. Inactivation of xcnM and xcnN eliminated conversion of Xcn1 to Xcn2 resulting in elevated Xcn1 production. The viability of the xcnM strain was reduced 20-fold relative to the wild-type strain supporting the idea that conversion of Xcn1 to Xcn2 provides a mechanism to avoid self-toxicity. Interestingly, inactivation of ompR enhanced cell viability during prolonged culturing.

Details

show
hide
Language(s):
 Dates: 2009
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: MOLECULAR MICROBIOLOGY
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 73 (5) Sequence Number: - Start / End Page: 938 - 949 Identifier: ISSN: 0950-382X