English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Biosynthesis of iso-fatty acids in myxobacteria

Dickschat, J., Bode, H. B., Kroppenstedt, R., Muller, R., & Schulz, S. (2005). Biosynthesis of iso-fatty acids in myxobacteria. ORGANIC & BIOMOLECULAR CHEMISTRY, 3(15), 2824-2831. doi:10.1039/b504889c.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Dickschat, JS1, Author
Bode, H. B.2, Author           
Kroppenstedt, RM1, Author
Muller, R1, Author
Schulz, S1, Author
Affiliations:
1external, ou_persistent22              
2Universität des Saarlandes, External Organization, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: The fatty acid (FA) profiles of the myxobacteria Stigmatella aurantiaca and Myxococcus xanthus were investigated by acidic methanolysis of total cell extracts and GC or GC-MS analysis. The main components were 13-methyltetradecanoic acid (iso-15:0) and (Z)-hexadec-11-enoic acid ( 16: 1, omega-5 cis). The biosynthesis of iso-FAs was investigated in several feeding experiments. Feeding of isovaleric acid (IVA) to a mutant impaired in the degradation of leucine to isovaleryl-CoA (IV-CoA) (bkd mutant) of M. xanthus only increased the amount of iso-odd FAs, whereas feeding of isobutyric acid ( IBA) gave increased amounts only of iso-even FAs. In contrast, a bkd mutant of S. aurantiaca gave increased amounts of iso-odd and iso-even fatty acids in both experiments. We assumed that in S. aurantiaca alpha-oxidation takes place. [D-7]-15-Methylhexadecanoic acid ( 8) was synthesised and fed to S. aurantiaca as well as [D-10] leucine and [D-8] valine to elucidate this pathway in more detail. The iso- fatty acid 8 was degraded by alpha- and beta-oxidation steps. [D-10] Leucine was strongly incorporated into iso-odd and iso-even fatty acids, whereas the incorporation rates for [D-8] valine into both types of fatty acids were low. Thus alpha-oxidation plays an important role in the biosynthesis of iso-fatty acids in S. aurantiaca. The incorporation rates observed after feeding of [D-10] leucine and [D-8] valine are the highest for iso-17: 0 compared to the other acids. This indicates the central role of iso-17 : 0 in the biosynthesis of iso-FAs. The shorter homologues seem to be formed mainly by alpha-oxidation and beta-oxidation of this acid. After feeding of 8 traces of unsaturated counterparts of this labelled FA occurred in the extracts indicating that desaturases are active in the biosynthesis of unsaturated fatty acids in S. aurantiaca.

Details

show
hide
Language(s):
 Dates: 2005
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000230602600017
DOI: 10.1039/b504889c
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ORGANIC & BIOMOLECULAR CHEMISTRY
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 3 (15) Sequence Number: - Start / End Page: 2824 - 2831 Identifier: ISSN: 1477-0520