English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Integrin αIIbβ3 activation and clustering in minimal synthetic cells

Benk, L. T., Benk, A. S., Lira, R. d., Cavalcanti-Adam, E. A., Dimova, R., Lipowsky, R., et al. (2022). Integrin αIIbβ3 activation and clustering in minimal synthetic cells. Advanced nanoBiomed research, 2(4): 2100094, pp. 1-9. doi:10.1002/anbr.202100094.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : Integrin anphaIIbbeta3 activation and clustering in minimal synthetic cells

Files

show Files
hide Files
:
AdvNanoBiomedRes_2_2022_2100094.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
AdvNanoBiomedRes_2_2022_2100094.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvNanoBiomedRes_2_2022_2100094_Suppl.pdf (Any fulltext), 3MB
 
File Permalink:
-
Name:
AdvNanoBiomedRes_2_2022_2100094_Suppl.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Creators

show
hide
 Creators:
Benk, Lucia T.1, Author              
Benk, Amelie S.1, Author              
Lira, Rafael de, Author
Cavalcanti-Adam, Elisabetta Ada1, Author              
Dimova, Rumiana, Author
Lipowsky, Reinhard, Author
Geiger, Benjamin, Author
Spatz, Joachim P.1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: cell adhesion; focal adhesion; integrin; integrin clustering; synthetic cell
 Abstract: Platelet adhesion and activation are mediated by integrin αIIbβ3 clustering, which is crucial for the hemostatic function of platelets. In an activated state, integrins provide the connection between the extracellular matrix and the actin cytoskeleton through a variety of cytoplasmic proteins, such as talin. Here, droplet-based microfluidics is applied to generate cell-sized giant unilamellar vesicles (GUVs) with a defined molecular composition to quantify the adhesion of integrin αIIbβ3-containing protocells in relation to the number of integrin–talin head domain (THD) complexes. Furthermore, it is shown that THD induces integrin clustering in protocells adhering to fibrinogen. The formation of this molecular link, which has, so far, only been observed in vivo, is an essential step in synthetic cell design to recapitulate integrin-mediated bidirectional signaling across the membrane. These results pave the way for further quantitative investigations of protein–protein interactions between integrins and associated proteins and their assembly within such defined, but complex, synthetic cells. An essential future step to mimic the complex interaction between cells and their environment will be to combine synthetic approaches with peptide chemistry to guide the molecular mechanisms involved in integrin binding and activation.

Details

show
hide
Language(s): eng - English
 Dates: 2022-01-272021-07-282022-02-20
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Advanced nanoBiomed research
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 2 (4) Sequence Number: 2100094 Start / End Page: 1 - 9 Identifier: ISSN: 2699-9307