English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Quantitative theory for the diffusive dynamics of liquid condensates.

Hubatsch, L., Jawerth, L., Love, C., Bauermann, J., Tang, T. D., Bo, S., et al. (2021). Quantitative theory for the diffusive dynamics of liquid condensates. eLife, 10: e68620. doi:10.7554/eLife.68620.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hubatsch, Lars1, Author           
Jawerth, Louise 1, Author           
Love, Celina1, Author           
Bauermann, Jonathan, Author
Tang, Ty Dora, Author
Bo, Stefano, Author
Hyman, Anthony1, Author           
Weber, Christoph A., Author
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

Content

show
hide
Free keywords: -
 Abstract: Key processes of biological condensates are diffusion and material exchange with their environment. Experimentally, diffusive dynamics are typically probed via fluorescent labels. However, to date, a physics-based, quantitative framework for the dynamics of labeled condensate components is lacking. Here we derive the corresponding dynamic equations, building on the physics of phase separation, and quantitatively validate the related framework via experiments. We show that by using our framework we can precisely determine diffusion coefficients inside liquid condensates via a spatio-temporal analysis of fluorescence recovery after photobleaching (FRAP) experiments. We showcase the accuracy and precision of our approach by considering space- and time-resolved data of protein condensates and two different polyelectrolyte-coacervate systems. Interestingly, our theory can also be used to determine a relationship between the diffusion coefficient in the dilute phase and the partition coefficient, without relying on fluorescence measurements in the dilute phase. This enables us to investigate the effect of salt addition on partitioning and bypasses recently described quenching artifacts in the dense phase. Our approach opens new avenues for theoretically describing molecule dynamics in condensates, measuring concentrations based on the dynamics of fluorescence intensities, and quantifying rates of biochemical reactions in liquid condensates.

Details

show
hide
Language(s):
 Dates: 2021-10-12
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.7554/eLife.68620
Other: cbg-8198
PMID: 34636323
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: eLife
  Other : Elife
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 10 Sequence Number: e68620 Start / End Page: - Identifier: -