English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Whole tissue and single cell mechanics are correlated in human brain tumors.

Sauer, F., Fritsch, A., Grosser, S., Pawlizak, S., Kießling, T., Reiss-Zimmermann, M., et al. (2021). Whole tissue and single cell mechanics are correlated in human brain tumors. Soft matter, 17(47), 10744-10752. doi:10.1039/d1sm01291f.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Sauer, Frank, Author
Fritsch, Anatol1, Author           
Grosser, Steffen, Author
Pawlizak, Steve, Author
Kießling, Tobias, Author
Reiss-Zimmermann, Martin, Author
Shahryari, Mehrgan, Author
Müller, Wolf C, Author
Hoffmann, Karl-Titus, Author
Käs, Josef A1, Author           
Sack, Ingolf, Author
Affiliations:
1Max Planck Institute for Molecular Cell Biology and Genetics, Max Planck Society, ou_2340692              

Content

show
hide
Free keywords: -
 Abstract: Biomechanical changes are critical for cancer progression. However, the relationship between the rheology of single cells measured ex-vivo and the living tumor is not yet understood. Here, we combined single-cell rheology of cells isolated from primary tumors with in vivo bulk tumor rheology in patients with brain tumors. Eight brain tumors (3 glioblastoma, 3 meningioma, 1 astrocytoma, 1 metastasis) were investigated in vivo by magnetic resonance elastography (MRE), and after surgery by the optical stretcher (OS). MRE was performed in a 3-Tesla clinical MRI scanner and magnitude modulus |G*|, loss angle φ, storage modulus G', and loss modulus G'' were derived. OS experiments measured cellular creep deformation in response to laser-induced step stresses. We used a Kelvin-Voigt model to deduce two parameters related to cellular stiffness (μKV) and cellular viscosity (ηKV) from OS measurements in a time regimen that overlaps with that of MRE. We found that single-cell μKV was correlated with |G*| (R = 0.962, p < 0.001) and G'' (R = 0.883, p = 0.004) but not G' of the bulk tissue. These results suggest that single-cell stiffness affects tissue viscosity in brain tumors. The observation that viscosity parameters of individual cells and bulk tissue were not correlated suggests that collective mechanical interactions (i.e. emergent effects or cellular unjamming) of many cancer cells, which depend on cellular stiffness, influence the mechanical dissipation behavior of the bulk tissue. Our results are important to understand the emergent rheology of active multiscale compound materials such as brain tumors and its role in disease progression.

Details

show
hide
Language(s):
 Dates: 2021-12-08
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1039/d1sm01291f
Other: cbg-8214
PMID: 34787626
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Soft matter
  Other : Soft Matter
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 17 (47) Sequence Number: - Start / End Page: 10744 - 10752 Identifier: -