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  Chapter Eight - A fast-track protocol for protein expression using the BEV system

Scholz, J., & Suppmann, S. (2021). Chapter Eight - A fast-track protocol for protein expression using the BEV system. In W. B. O’Dell, & Z. Kelman (Eds.), Recombinant Protein Expression: Eukaryotic Hosts (pp. 171-190). London: Academic Pr.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-23A4-5 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-ABF2-4
Genre: Book Chapter
Other : A fast-track protocol for protein expression using the BEV system

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 Creators:
Scholz, Judith1, Author           
Suppmann, Sabine1, Author           
Affiliations:
1Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: INSECT-CELLBiochemistry & Molecular Biology; Biotechnology & Applied Microbiology;
 Abstract: Baculovirus-insect cell expression (BEV) has become one of the most widely used eukaryotic systems for heterologous protein expression. The combination of engineered baculovirus genomes together with a variety of compatible vectors, robust insect cell lines, serum-free media and commercial kits have made it a standard workhorse in many "non-virology-expert" laboratories. Despite these significant improvements, the BEV system still has major drawbacks, primarily the time required to amplify recombinant virus and its inherent instability. Here we present an easy-to-adopt simplified and shortened protocol.

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Language(s): eng - English
 Dates: 2021
 Publication Status: Issued
 Pages: 20
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000750883800009
DOI: 10.1016/bs.mie.2021.06.015
 Degree: -

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Title: Recombinant Protein Expression: Eukaryotic Hosts
  Alternative Title : Methods Enzymol.
Source Genre: Book
 Creator(s):
O’Dell, William B.1, Editor
Kelman, Zvi 1, Editor
Affiliations:
1 External Organizations, ou_persistent22            
Publ. Info: London : Academic Pr
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 171 - 190 Identifier: ISSN: 0076-6879
ISBN: 978-0-323-90737-8

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Title: Methods in Enzymology
  Alternative Title : METHOD ENZYMOL
  Alternative Title : Methods Enzymol.
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 660 Sequence Number: - Start / End Page: - Identifier: ISSN: 0076-6879