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  Studying the Key Intermediate of RNA Autohydrolysis by Cryogenic Gas-Phase Infrared Spectroscopy

Greis, K., Kirschbaum, C., Taccone, M., Götze, M., Gewinner, S., Schöllkopf, W., et al. (2022). Studying the Key Intermediate of RNA Autohydrolysis by Cryogenic Gas-Phase Infrared Spectroscopy. Angewandte Chemie International Edition, 61(19): e202115481. doi:10.1002/anie.202115481.

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Angew Chem Int Ed - 2022 - Greis - Studying the Key Intermediate of RNA Autohydrolysis by Cryogenic Gas%u2010Phase Infrared.pdf (Publisher version), 5MB
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Angew Chem Int Ed - 2022 - Greis - Studying the Key Intermediate of RNA Autohydrolysis by Cryogenic Gas%u2010Phase Infrared.pdf
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 Creators:
Greis, Kim1, 2, Author           
Kirschbaum, Carla1, 2, Author           
Taccone, Martin2, Author           
Götze, Michael1, 2, Author
Gewinner, Sandy2, Author           
Schöllkopf, Wieland2, Author           
Meijer, Gerard2, Author           
Helden, Gert von2, Author           
Pagel, Kevin1, 2, Author           
Affiliations:
1Institut für Chemie und Biochemie, Freie Universität Berlin, Arnimallee 22, 14195 Berlin, Germany , ou_persistent22              
2Molecular Physics, Fritz Haber Institute, Max Planck Society, ou_634545              

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 Abstract: Over the course of the COVID-19 pandemic, mRNA-basedvaccineshave gained tremendous importance. The development and analysis of modified RNA moleculesbenefit from advanced mass spectrometry and require sufficient understanding of fragmentation processes.Analogous tothe degradation of RNA in solution by autohydrolysis,backbone cleavage of RNA strands wasequally observedin the gas phase; however, the fragmentation mechanism remained elusive.In this work,autohydrolysis-like intermediates weregenerated from isolated RNA dinucleotidesin the gas phaseand investigatedusing cryogenic infrared spectroscopy in helium nanodroplets.Data from both experiment and density functional theory provide evidence forthe formation of a five-membered cyclic phosphateintermediateand rule outlinear orsix-membered structures. Furthermore, the experiments show that another prominent condensed-phase reactionof RNA nucleotides can be induced in the gas phase: the tautomerization of cytosine.Both observed reactions aretherefore highlyuniversal and intrinsic properties of the investigated molecules.

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Language(s): eng - English
 Dates: 2021-11-142022-03-012022-03-012022-05-02
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.202115481
 Degree: -

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Project name : GlycoSpec - Unravelling Glycochemistry with Ion Mobility Spectrometry and Gas-Phase Spectroscopy
Grant ID : 863934
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Angewandte Chemie International Edition
  Abbreviation : Angew. Chem., Int. Ed.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: 7 Volume / Issue: 61 (19) Sequence Number: e202115481 Start / End Page: - Identifier: ISSN: 1433-7851
CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851