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2DE-MS, bottom-up MS, protein speciation, protein species, top-down MS
Abstract:
In contrast to bottom-up LC-MS only 2DE-MS can separate and detect a huge number of human protein species. Kwiatkowski et al. (in this issue) established parameters to estimate the amount of protein speciation for each human protein. Proteins identified in 2DE-MS approaches showed more protein speciation than in bottom-up LC-MS. The authors state that protein speciation is likely to increase the chance of proteins to be determined in 2-DE/MS, though admitting that low sensitivity 2DE-MS methods were used in this study. In agreement with Kwiatkowski et al. we are convinced, this difference between 2DE-MS and bottom-up LC-MS will disappear, if high-resolution 2DE, is combined with identification by a high-sensitivity LC-Orbitrap-MS. Meta-analysis of proteomic data is surely a promising tool, though the technological progress in 2DE and MS has to reach a plateau to enable useful comparisons. This article is protected by copyright. All rights reserved.
