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  Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3

Upmanyu, N., Jin, J., Emde, H. v. d., Ganzella, M., Bösche, L., Malviya, V. N., et al. (2022). Colocalization of different neurotransmitter transporters on synaptic vesicles is sparse except for VGLUT1 and ZnT3. Neuron, In Press. doi:10.1016/j.neuron.2022.02.008.

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 Creators:
Upmanyu, N.1, Author
Jin, J., Author
Emde, H. von der2, Author
Ganzella, M.3, Author
Bösche, L.1, Author
Malviya, V. N.3, Author
Zhuleku, E., Author
Politi, A. Z.4, Author
Ninov, M.5, Author
Silbern, I.5, Author
Leutenegger, M.2, Author
Urlaub, H.5, Author
Riedel, D.6, Author
Preobraschenski, J.3, Author
Milosevic, I., Author
Hell, S. W.2, Author
Jahn, R.3, Author
Sambandan, S.1, Author
Affiliations:
1Synaptic Metal Ion Dynamics and Signaling, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_persistent22              
2Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, Göttingen, DE, ou_3350048              
3Laboratory of Neurobiology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_persistent22              
4Facility for Live-Cell Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, Göttingen, DE, ou_3371546              
5Research Group of Bioanalytical Mass Spectrometry, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, Göttingen, DE, ou_3350290              
6Department of Structural Dynamics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, Göttingen, DE, ou_3350272              

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Free keywords: synaptic vesicle; vesicular transporters; VGLUT1; zinc transporter 3; ZnT3; DyMIN STED; MINSTED; zinc; glutamate; super resolution microscopy
 Abstract: Vesicular transporters (VTs) define the type of neurotransmitter that synaptic vesicles (SVs) store and release. While certain mammalian neurons release multiple transmitters, it is not clear whether the release occurs from the same or distinct vesicle pools at the synapse. Using quantitative single-vesicle imaging, we show that a vast majority of SVs in the rodent brain contain only one type of VT, indicating specificity for a single neurotransmitter. Interestingly, SVs containing dual transporters are highly diverse (27 types) but small in proportion (2% of all SVs), excluding the largest pool that carries VGLUT1 and ZnT3 (34%). Using VGLUT1-ZnT3 SVs, we demonstrate that the transporter colocalization influences the SV content and synaptic quantal size. Thus, the presence of diverse transporters on the same vesicle is bona fide, and depending on the VT types, this may act to regulate neurotransmitter type, content, and release in space and time.

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Language(s): eng - English
 Dates: 2022-03-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.neuron.2022.02.008
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Title: Neuron
Source Genre: Journal
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Pages: 15. e7 Volume / Issue: - Sequence Number: In Press Start / End Page: - Identifier: ISSN: 08966273