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  MINSTED nanoscopy enters the Ångström localization range

Weber, M., von der Emde, H., Leutenegger, M., Gunkel, P., Sambandan, S., Khan, T. A., et al. (2022). MINSTED nanoscopy enters the Ångström localization range. Nature Biotechnology. doi:10.1038/s41587-022-01519-4.

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 Creators:
Weber, M.1, Author           
von der Emde, H.1, Author           
Leutenegger, Marcel1, Author           
Gunkel, P.2, Author           
Sambandan, S.3, Author           
Khan, T. A.1, Author           
Keller-Findeisen, J.1, Author           
Cordes, Volker C.2, Author           
Hell, S. W.1, Author                 
Affiliations:
1Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, Göttingen, DE, ou_3350048              
2Department of Cellular Logistics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, Göttingen, DE, ou_3350135              
3Emeritus Group Laboratory of Neurobiology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350145              

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 Abstract: Super-resolution techniques have achieved localization precisions in the nanometer regime. Here we report all-optical, room temperature localization of fluorophores with precision in the Ångström range. We built on the concept of MINSTED nanoscopy where precision is increased by encircling the fluorophore with the low-intensity central region of a stimulated emission depletion (STED) donut beam while constantly increasing the absolute donut power. By blue-shifting the STED beam and separating fluorophores by on/off switching, individual fluorophores bound to a DNA strand are localized with σ = 4.7 Å, corresponding to a fraction of the fluorophore size, with only 2,000 detected photons. MINSTED fluorescence nanoscopy with single-digit nanometer resolution is exemplified by imaging nuclear pore complexes and the distribution of nuclear lamin in mammalian cells labeled by transient DNA hybridization. Because our experiments yield a localization precision σ = 2.3 Å, estimated for 10,000 detected photons, we anticipate that MINSTED will open up new areas of application in the study of macromolecular complexes in cells.

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Language(s): eng - English
 Dates: 2022-11-07
 Publication Status: Published online
 Pages: -
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 Table of Contents: -
 Rev. Type: No review
 Identifiers: DOI: 10.1038/s41587-022-01519-4
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Title: Nature Biotechnology
  Abbreviation : Nat. Biotechnol.
Source Genre: Journal
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Publ. Info: New York : Gale Group Inc.
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 1087-0156
CoNE: https://pure.mpg.de/cone/journals/resource/954926980065