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  Isotope analysis of human dental calculus δ13CO32-: investigating a potential new proxy for sugar consumption

Chidimuro, B., Mundorff, A., Speller, C., Radini, A., Boudreault, N., Lucas, M., et al. (2022). Isotope analysis of human dental calculus δ13CO32-: investigating a potential new proxy for sugar consumption. Rapid Communications in Mass Spectrometry, e9286. doi:10.1002/rcm.9286.

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Figure S1, Table S1-S5 (Supplementary material)
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 Creators:
Chidimuro, Blessing, Author
Mundorff, Amy, Author
Speller, Camilla, Author
Radini, Anita, Author
Boudreault, Noémie, Author
Lucas, Mary1, Author              
Holst, Malin, Author
Lamb, Angela, Author
Collins, Matthew, Author
Alexander, Michelle, Author
Affiliations:
1Archaeology, Max Planck Institute for the Science of Human History, Max Planck Society, ou_2074312              

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Free keywords: Isotope analysis, Dental calculus, Bone, Enamel, C4 Diet
 Abstract: RATIONALE: Dental calculus (mineralized dental plaque) is composed primarily of hydroxyapatite. We hypothesize that the carbonate component of dental calculus will reflect the isotopic composition of ingested simple carbohydrates. Therefore, dental calculus carbonates may be an indicator for sugar consumption, and an alternative to bone carbonate in isotopic paleodiet studies. METHODS We utilised FTIR-ATR analysis to characterise the composition and crystallisation of bone and dental calculus before isotope analysis of carbonate. Using a Sercon 20-22 mass spectrometer coupled with a Sercon GSL Sample Preparation System and an IsoPrime 100 dual inlet mass spectrometer plus Multiprep device to measure carbon, we tested the potential of dental calculus carbonate to identify C4 resources in diet through analysis of δ13C values in paired bone, calculus, and teeth mineral samples. - RESULTS: The modern population shows higher δ13C values in all three tissue carbonates compared to both archaeological populations. Clear differences in dental calculus δ13C values are observed between the modern and archaeological individuals suggesting potential for utilising dental calculus in isotope paleodiet studies. The offset between dental calculus and either bone or enamel carbonate δ13C values are large and consistent in direction, with no consistent offset between the δ13C values for the three tissues per individual. - CONCLUSIONS: Our results support dental calculus carbonate as a new biomaterial to identify C4 sugar through isotope analysis. Greater carbon fractionation in the mouth is likely due to the complex formation of dental calculus as a mineralized biofilm, which results in consistently high δ13C values compared to bone and enamel.

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Language(s): eng - English
 Dates: 2022-03-08
 Publication Status: Published online
 Pages: 29
 Publishing info: -
 Table of Contents: 1 Introduction
1.1 Dental Calculus and potential for isotopic analysis
2 Mterial and methods
2.1 Materials
2.2 Methods
2.2.1 FTIR-ATR
2.2.2 Bone collagen extraction
2.2.3 Bone preparation for carbonate analysis
2.2.4 Enamel preparation for carbonate analysis
2.2.5 Dental calculus preparation for carbonate analysis
2.3 Analytical measurement
2.3.1 Bone collagen
2.3.2 Bone, enamel, and dental calculus carbonates
2.4 Suess effect correction
2.5 Statistical analysis
3 Results and discussion
3.1 Bone and dental calculus FTIR-ATR
3.1.1 Infrared splitting factor (IRSF)
3.1.2 Carbonate-phosphate ratio (C/P)
3.1.3 IRSF and C/P relationship
3.2 Isotopes
3.2.1 Correlations between tissues
3.2.2 Carbonate δ13C tissue offsets
3.2.3 Dietary interpretation from the three tissues
3.2.4 Causes of diet differences between bone and enamel
4 Conclusions
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/rcm.9286
Other: shh3165
 Degree: -

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Title: Rapid Communications in Mass Spectrometry
  Other : Rapid Commun. Mass Spectrom.
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: - Sequence Number: e9286 Start / End Page: - Identifier: ISSN: 0951-4198
CoNE: https://pure.mpg.de/cone/journals/resource/954925574961