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  The promoting effects of activated olfactory ensheathing cells on angiogenesis after spinal cord injury through the PI3K/Akt pathway

Wang, X., Jiang, C., Zhang, Y., Chen, Z., Fan, H., Zhang, Y., et al. (2022). The promoting effects of activated olfactory ensheathing cells on angiogenesis after spinal cord injury through the PI3K/Akt pathway. CELL AND BIOSCIENCE, 12(1): 23. doi:10.1186/s13578-022-00765-y.

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 Creators:
Wang, Xiaohui1, Author              
Jiang, Chao, Author
Zhang, Yongyuan, Author
Chen, Zhe, Author
Fan, Hong, Author
Zhang, Yuyang, Author
Wang, Zhiyuan, Author
Tian, Fang, Author
Li, Jing, Author
Yang, Hao, Author
Hao, Dingjun, Author
Affiliations:
1Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591697              

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 Abstract: Objective The aim of this study was to investigate the pro-angiogenic potential of olfactory ensheathing cells (OECs) activated by curcumin (CCM) and lipopolysaccharide (LPS) and the possible underlying mechanisms. Methods Vascular endothelial cells or tissues were cultured and treated with conditioned medium (CM) extracted from activated OECs activated through the addition of LPS and CCM or unactivated controls. Concomitantly, the pro-angiogenic potential of OECs was assessed in vitro by aortic ring sprouting assay, endothelial wound healing assay, CCK-8 assay, and tube formation assay. Subsequently, the OECs were co-cultured with endothelial cells to evaluate their promoting effect on endothelial cell proliferation and migration following a mechanical scratch. Moreover, the spinal cord injury (SCI) model in rats was established, and the number of endothelial cells and vascular structure in the injured area after SCI was observed with OEC transplantation. Finally, the underlying mechanism was investigated by western blot analysis of phosphorylated kinase expression with or without the MK-2206 (Akt-inhibitor). Result The present results showed that the activated OECs can effectively promote vascular endothelial cells' proliferation, migration, and vessel-like structure formation. Strikingly, several pro-angiogenic growth factors such as VEGF-A and PDGF-AA, which facilitate vessel formation, were found to be significantly elevated in CM. In addition, the PI3K/Akt signaling pathway was found to be involved in pro-angiogenic events caused by activated OEC CM, displaying higher phosphorylation levels in cells. In contrast, the delivery of MK2206 can effectively abrogate all the positive effects. Conclusions OECs activated by LPS and CCM have a pro-angiogenic effect and can effectively promote angiogenesis and improve the microenvironment at the injury site when transplanted in the injured spinal cord. This potentiated ability of OECs to provide pro-angiogenic effects is likely mediated through the PI3K/Akt pathway.

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 Dates: 2022-03-04
 Publication Status: Published online
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 Identifiers: ISI: 000764770100001
DOI: 10.1186/s13578-022-00765-y
PMID: 35246244
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Title: CELL AND BIOSCIENCE
Source Genre: Journal
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Pages: - Volume / Issue: 12 (1) Sequence Number: 23 Start / End Page: - Identifier: -