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  Sialylated N-glycans mediate monocyte uptake of extracellular vesicles secreted from Plasmodium falciparum-infected red blood cells

Ben Ami Pilo, H., Khilji, S. K., Lühle, J., Biskup, K., Levy Gal, B., Rosenhek Goldian, I., et al. (2022). Sialylated N-glycans mediate monocyte uptake of extracellular vesicles secreted from Plasmodium falciparum-infected red blood cells. Journal of Extracellular Biology, 1(2): e33. doi:10.1002/jex2.33.

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 Creators:
Ben Ami Pilo, Hila, Author
Khilji, Sana K.1, Author              
Lühle, Jost, Author
Biskup, Karina, Author
Levy Gal, Bar, Author
Rosenhek Goldian, Irit, Author
Alfandari, Daniel, Author
Revach, Or-Yam, Author
Kiper, Edo, Author
Morandi, Mattia I., Author
Rotkopf, Ron, Author
Porat, Ziv, Author
Blanchard, Véronique, Author
Seeberger, Peter H.2, Author              
Regev-Rudzki, Neta, Author
Moscovitz, Oren1, Author              
Affiliations:
1Oren Moscovitz, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_3176803              
2Peter H. Seeberger - Vaccine Development, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863308              

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 Abstract: Glycoconjugates on extracellular vesicles (EVs) play a vital role in internalization and mediate interaction as well as regulation of the host immune system by viruses, bacteria, and parasites. During their intraerythrocytic life-cycle stages, malaria parasites, Plasmodium falciparum (Pf) mediate the secretion of EVs by infected red blood cells (RBCs) that carry a diverse range of parasitic and host-derived molecules. These molecules facilitate parasite-parasite and parasite-host interactions to ensure parasite survival. To date, the number of identified Pf genes associated with glycan synthesis and the repertoire of expressed glycoconjugates is relatively low. Moreover, the role of Pf glycans in pathogenesis is mostly unclear and poorly understood. As a result, the expression of glycoconjugates on Pf-derived EVs or their involvement in the parasite life-cycle has yet to be reported. Herein, we show that EVs secreted by Pf-infected RBCs carry significantly higher sialylated complex N-glycans than EVs derived from healthy RBCs. Furthermore, we reveal that EV uptake by host monocytes depends on N-glycoproteins and demonstrate that terminal sialic acid on the N-glycans is essential for uptake by human monocytes. Our results provide the first evidence that Pf exploits host sialylated N-glycans to mediate EV uptake by the human immune system cells.

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Language(s): eng - English
 Dates: 2022-03-212022
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/jex2.33
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Title: Journal of Extracellular Biology
Source Genre: Journal
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Publ. Info: Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles
Pages: - Volume / Issue: 1 (2) Sequence Number: e33 Start / End Page: - Identifier: ISSN: 2768-2811