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  Bottom-up assembly of functional DNA-based cytoskeletons for synthetic cells

Jahnke, K., Zhan, Pengfei, P., Liu, N., & Gopfrich, K. (2022). Bottom-up assembly of functional DNA-based cytoskeletons for synthetic cells. Biophysical Journal (Annual Meeting Abstracts), 121(3), 151A-151A. doi:10.1016/j.bpj.2021.11.1964.

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BiophysJ_121_2022_S1_151a.pdf (Any fulltext), 38KB
 
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Jahnke, Kevin1, Author           
Zhan, Pengfei, Pengfei, Author
Liu, Na, Author
Gopfrich, Kerstin1, Author           
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1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: Bottom-up synthetic biology aims at reconstructing a cell from biomolecular constituents. However, the combination of multiple elements and functions remained elusive, which stimulates endeavors to explore entirely synthetic bio-inspired solutions towards engineering life. To this end, DNA nanotechnology represents one of the most promising routes, given the inherent sequence specificity, addressability, and programmability of DNA. Here, we demonstrate functional DNA-based cytoskeletons operating in microfluidic cell-sized compartments and lipid vesicles. The synthetic cytoskeletons consist of DNA tiles self-assembled into filament networks. These filaments can be rationally designed and controlled to imitate features of natural cytoskeletons, including dynamic instability, ATP-triggered polymerization, and vesicle transport in cell-sized confinement. Also, they possess engineerable characteristics, including assembly and disassembly powered by DNA hybridization, light or aptamer-target interactions and autonomous transport of gold nanoparticles. This work underpins DNA nanotechnology as a key player in building synthetic cells.

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Language(s): eng - English
 Dates: 2022-02-11
 Publication Status: Issued
 Pages: 1
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.bpj.2021.11.1964
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Title: Biophysical Journal (Annual Meeting Abstracts)
  Other : Biophys. J. (Annual Meeting Abstracts)
Source Genre: Journal
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Publ. Info: Bethesda, MD : Biophysical Society
Pages: - Volume / Issue: 121 (3) Sequence Number: - Start / End Page: 151A - 151A Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117_1