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  Proteome profile of peripheral myelin in healthy mice and in a neuropathy model

Siems, S. B., Jahn, O., Eichel, M. A., Kannaiyan, N., Wu, L. M. N., Sherman, D. L., et al. (2020). Proteome profile of peripheral myelin in healthy mice and in a neuropathy model. eLife, 9: 51406. doi:10.7554/eLife.51406.

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 Creators:
Siems, S. B.1, Author           
Jahn, O.2, Author           
Eichel, M. A.1, Author           
Kannaiyan, N., Author
Wu, L. M. N., Author
Sherman, D. L., Author
Kusch, K.1, Author           
Hesse, D.2, Author           
Jung, R. B.1, Author
Fledrich, R.1, Author           
Sereda, M. W.1, Author           
Rossner, M. J., Author
Brophy, P. J., Author
Werner, H. B.1, Author           
Affiliations:
1Neurogenetics, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173664              
2Proteomics, Wiss. Servicegruppen, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173673              

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Free keywords: charcot-marie-tooth (cmt) neuropathy; demyelination; mouse; myelin proteome; neuroscience; peripheral nervous system; schwann cell.
 Abstract: Proteome and transcriptome analyses aim at comprehending the molecular profiles of the brain, its cell-types and subcellular compartments including myelin. Despite the relevance of the peripheral nervous system for normal sensory and motor capabilities, analogous approaches to peripheral nerves and peripheral myelin have fallen behind evolving technical standards. Here we assess the peripheral myelin proteome by gel-free, label-free mass-spectrometry for deep quantitative coverage. Integration with RNA-Sequencing-based developmental mRNA-abundance profiles and neuropathy disease genes illustrates the utility of this resource. Notably, the periaxin- deficient mouse model of the neuropathy Charcot-Marie-Tooth 4F displays a highly pathological myelin proteome profile, exemplified by the discovery of reduced levels of the monocarboxylate transporter MCT1/SLC16A1 as a novel facet of the neuropathology. This work provides the most comprehensive proteome resource thus far to approach development, function and pathology of peripheral myelin, and a straightforward, accurate and sensitive workflow to address myelin diversity in health and disease.

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Language(s): eng - English
 Dates: 2020-03-04
 Publication Status: Published online
 Pages: 31
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.7554/eLife.51406
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Project name : Deutsche Forschungsge- meinschaft (DFG) WE 2720/2-2, Deutsche Forschungsgemeinschaft (DFG) WE 2720/4-1, Deutsche Forschungsge- meinschaft (DFG) WE 2720/5-1, Deutsche Forschungsge- meinschaft (DFG) RO 4076/3-2,
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Grant ID : 0842424
Funding program : Wellcome Trust Funding
Funding organization : Wellcome Trust (WT)

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Title: eLife
Source Genre: Journal
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Pages: 31 Volume / Issue: 9 Sequence Number: 51406 Start / End Page: - Identifier: ISSN: 2050-084X