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  Río‐Hortega's drawings revisited with fluorescent protein defines a cytoplasm‐filled channel system of CNS myelin

Edgar, J. M., McGowan, E., Chapple, K. J., Möbius, W., Lemgruber, L., Insall, R. H., et al. (2021). Río‐Hortega's drawings revisited with fluorescent protein defines a cytoplasm‐filled channel system of CNS myelin. Journal of Anatomy, 239(6), 1241-1255. doi:10.1111/joa.13577.

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 Creators:
Edgar, J. M.1, Author           
McGowan, E., Author
Chapple, K. J., Author
Möbius, W.1, 2, Author           
Lemgruber, L., Author
Insall, R. H., Author
Nave, K.-A.1, Author           
Boullerne, A., Author
Affiliations:
1Neurogenetics, Max Planck Institute of Experimental Medicine, Max Planck Society, ou_2173664              
2Electron microscopy, Neurogenetics, Max Planck Institute of Experimental Medicine, Max Planck Society, Hermann-Rein-Str. 3, 37075 Göttingen, DE, ou_2173666              

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Free keywords: history; live-imaging; non-compact myelin; silver carbonate staining.
 Abstract: A century ago this year, Pío del Río-Hortega (1921) coined the term ‘oligodendroglia’ for the ‘interfascicular glia’ with very few processes, launching an extensive discovery effort on his new cell type. One hundred years later, we review his original contribu- tions to our understanding of the system of cytoplasmic channels within myelin in the context of what we observe today using light and electron microscopy of genetically encoded fluorescent reporters and immunostaining. We use the term myelinic channel system to describe the cytoplasm-delimited spaces associated with myelin; being the paranodal loops, inner and outer tongues, cytoplasm-filled spaces through compact myelin and further complex motifs associated to the sheath. Using a central nervous system myelinating cell culture model that contains all major neural cell types and produces compact myelin, we find that td-tomato fluorescent protein delineates the myelinic channel system in a manner reminiscent of the drawings of adult white mat - ter by Río-Hortega, despite that he questioned whether some cytoplasmic figures he observed represented artefact. Together, these data lead us to propose a slightly revised model of the ‘unrolled’ sheath. Further, we show that the myelinic channel system, while relatively stable, can undergo subtle dynamic shape changes over days. Importantly, we capture an under-appreciated complexity of the myelinic channel sys- tem in mature myelin sheaths.

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Language(s): eng - English
 Dates: 2021-10-112021-10-282021-12
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1111/joa.13577
 Degree: -

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Project name : We are grateful to the UK MS Society (Grant refs 38 and 127 to JE), NIH/National Library of Medicine (grant G13LM011465 to AB), the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) TRR 274/1 2020—408885537 to KAN), Research Center Nanoscale Microscopy and Molecular Physiology of the Brain, CNMPB (to KAN and WM) as well as the European Research Council (ERC, erc.europa.eu: ERC Advanced Grants AxoGlia and MyeliNANO to KAN).
Grant ID : -
Funding program : -
Funding organization : -
Project name : MyeliNano
Grant ID : 671048
Funding program : Horizon 2020 (H2020)
Funding organization : European Commission (EC)

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Title: Journal of Anatomy
Source Genre: Journal
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Publ. Info: Wiley
Pages: - Volume / Issue: 239 (6) Sequence Number: - Start / End Page: 1241 - 1255 Identifier: ISSN: 0021-8782
ISSN: 1469-7580