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  Transplastomic approaches for metabolic engineering

Bock, R. (2022). Transplastomic approaches for metabolic engineering. Current Opinion in Plant Biology, 66: 102185. doi:10.1016/j.pbi.2022.102185.

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 Creators:
Bock, R.1, Author              
Affiliations:
1Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: Plastid transformation, Chloroplast transformation, Metabolic engineering, Synthetic biology, Synthetic operon, Inducible expression, Isoprenoid metabolism, Carotenoid biosynthesis, Astaxanthin
 Abstract: The plastid (chloroplast) genome of seed plants represents an attractive target of metabolic pathway engineering by genetic transformation. Although the plastid genome is relatively small, it can accommodate large amounts of foreign DNA that precisely integrates via homologous recombination, and is largely excluded from pollen transmission due to the maternal mode of plastid inheritance. Since the engineering of metabolic pathways often requires the expression of multiple transgenes, the possibility to conveniently stack transgenes in synthetic operons makes the transplastomic technology particularly appealing in the area of metabolic engineering. Absence of epigenetic gene silencing mechanisms from plastids and the possibility to achieve high transgene expression levels further add to the attractiveness of plastid genome transformation. This review focuses on engineering principles and available tools for the transplastomic expression of enzymes and pathways, and highlights selected recent applications in metabolic engineering.

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Language(s): eng - English
 Dates: 2022-02-172022-04
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1016/j.pbi.2022.102185
 Degree: -

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Title: Current Opinion in Plant Biology
  Other : Curr. Opin. Plant Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London : Elsevier Current Trends
Pages: 102185 Volume / Issue: 66 Sequence Number: 102185 Start / End Page: - Identifier: ISSN: 1369-5266
CoNE: https://pure.mpg.de/cone/journals/resource/954925620174