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  Impaired KIN10 function restores developmental defects in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutant

Zacharaki, V., Ponnu, J., Crepin, N., Langenecker, T., Hagmann, J., Skorzinski, N., et al. (2022). Impaired KIN10 function restores developmental defects in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutant. New Phytologist, 235(1), 220-233. doi:10.1111/nph.18104.

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Genre: Journal Article
Alternative Title : New Phytologist

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 Creators:
Zacharaki, Vasiliki1, Author
Ponnu, Jathish1, Author
Crepin, Nathalie1, Author
Langenecker, Tobias1, Author
Hagmann, Jörg1, Author
Skorzinski, Noemi1, Author
Musialak-Lange, M.2, Author           
Wahl, V.2, Author           
Rolland, Filip1, Author
Schmid, Markus1, Author
Affiliations:
1external, ou_persistent22              
2Metabolism and Development, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2435692              

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Free keywords: Arabidopsis thaliana, embryogenesis, flowering time, SnRK1 complex, T6P pathway, TPS1
 Abstract: Summary Sensing carbohydrate availability is essential for plants to coordinate their growth and development. In Arabidopsis thaliana, TREHALOSE 6-PHOSPHATE SYNTHASE 1 (TPS1) and its product, trehalose 6-phosphate (T6P), are important for the metabolic control of development. tps1 mutants are embryo lethal and unable to flower when embryogenesis is rescued. T6P regulates development in part through inhibition of SUCROSE NON-FERMENTING1 RELATED KINASE1 (SnRK1). Here, we explored the role of SnRK1 in T6P-mediated plant growth and development using a combination of a mutant suppressor screen and genetic, cellular, and transcriptomic approaches. We report non-synonymous amino acid substitutions in the catalytic KIN10 and regulatory SNF4 subunits of SnRK1 that can restore both embryogenesis and flowering of tps1 mutant plants. The identified SNF4 point mutations disrupt the interaction with the catalytic subunit KIN10. Contrary to the common view that the two A. thaliana SnRK1 catalytic subunits act redundantly, we found that loss-of-function mutations in KIN11 are unable to restore embryogenesis and flowering, highlighting the important role of KIN10 in T6P signalling.

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Language(s): eng - English
 Dates: 2022-03-20
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1111/nph.18104
 Degree: -

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Title: New Phytologist
  Other : New Phytol.
Source Genre: Journal
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Publ. Info: Wiley; New Phytologist Foundation
Pages: - Volume / Issue: 235 (1) Sequence Number: - Start / End Page: 220 - 233 Identifier: ISSN: 0028-646X
CoNE: https://pure.mpg.de/cone/journals/resource/954925334695