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  Resistance to RNA interference by plant-derived double-stranded RNAs but not plant-derived short interfering RNAs in Helicoverpa armigera

Fu, J., Xu, S., Lu, H., Li, F., Li, S., Chang, L., et al. (2022). Resistance to RNA interference by plant-derived double-stranded RNAs but not plant-derived short interfering RNAs in Helicoverpa armigera. Plant, Cell and Environment. doi:10.1111/pce.14314.

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Genre: Journal Article
Alternative Title : Plant, Cell & Environment

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 Creators:
Fu, Jinqiu1, Author
Xu, Shijing1, Author
Lu, Huan1, Author
Li, Fanchi1, Author
Li, Shengchun1, Author
Chang, Ling1, Author
Heckel, David G.1, Author
Bock, R.2, Author           
Zhang, Jiang1, Author
Affiliations:
1external, ou_persistent22              
2Organelle Biology and Biotechnology, Department Bock, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753326              

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Free keywords: cotton bollworm, double-stranded RNA, plastid transformation, RNA interference, short interfering RNA, transgenic plants
 Abstract: Abstract Plant-mediated RNA interference (RNAi) has emerged as a promising technology for pest control through expression of double-stranded RNAs (dsRNAs) targeted against essential insect genes. However, little is known about the underlying molecular mechanisms and whether long dsRNA or short interfering RNAs (siRNAs) are the effective triggers of the RNAi response. Here we generated transplastomic and nuclear transgenic tobacco plants expressing dsRNA against the Helicoverpa armigera ATPaseH gene. We showed that expression of long dsRNA of HaATPaseH was at least three orders of magnitude higher in transplastomic plants than in transgenic plants. HaATPaseH-derived siRNAs are absent from transplastomic plants, while they are abundant in transgenic plants. Feeding transgenic plants to H. armigera larvae reduced gene expression of HaATPaseH and delayed growth. Surprisingly, no effect of transplastomic plants on insect growth was observed, despite efficient dsRNA expression in plastids. Furthermore, we found that dsRNA ingested by H. armigera feeding on transplastomic plants was rapidly degraded in the intestinal fluid. In contrast, siRNAs are relatively stable in the digestive system. These results suggest that plant-derived siRNAs may be more effective triggers of RNAi in Lepidoptera than dsRNAs, which will aid the optimization of the strategies for plant-mediated RNAi to pest control. This article is protected by copyright. All rights reserved.

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Language(s): eng - English
 Dates: 2022-03-212022-03-21
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1111/pce.14314
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Title: Plant, Cell and Environment
  Other : Plant, Cell & Environment
Source Genre: Journal
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Publ. Info: Oxford, England : Blackwell Science
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: - Identifier: ISSN: 0140-7791
CoNE: https://pure.mpg.de/cone/journals/resource/954925471334