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  Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction

Zywitza, V., Rusha, E., Shaposhnikov, D., Ruiz-Orera, J., Telugu, N., Rishko, V., et al. (2022). Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction. Scientific Reports, 12: 3100. doi:10.1038/s41598-022-07059-w.

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ScientificReports_Zywitza et al_2022.pdf (Publisher version), 20MB
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Zywitza, Vera , Author
Rusha, Ejona, Author
Shaposhnikov, Dmitry , Author
Ruiz-Orera, Jorge , Author
Telugu, Narasimha , Author
Rishko, Valentyna , Author
Hayashi, Masafumi , Author
Michel, Geert , Author
Wittler, Lars1, Author              
Stejskal, Jan, Author
Holtze, Susanne , Author
Göritz, Frank , Author
Hermes, Robert, Author
Wang, Jichang , Author
Izsvák, Zsuzsanna , Author
Colleoni, Silvia , Author
Lazzari, Giovanna, Author
Galli, Cesare , Author
Hildebrandt, Thomas B. , Author
Hayashi, Katsuhiko , Author
Diecke, Sebastian, AuthorDrukker, Micha, Author more..
Affiliations:
1Dept. of Developmental Genetics (Head: Bernhard G. Herrmann), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433548              

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Free keywords: Biological techniques, Cell biology, Developmental biology, Stem cells
 Abstract: The northern white rhinoceros (NWR) is probably the earth’s most endangered mammal. To rescue the functionally extinct species, we aim to employ induced pluripotent stem cells (iPSCs) to generate gametes and subsequently embryos in vitro. To elucidate the regulation of pluripotency and differentiation of NWR PSCs, we generated iPSCs from a deceased NWR female using episomal reprogramming, and observed surprising similarities to human PSCs. NWR iPSCs exhibit a broad differentiation potency into the three germ layers and trophoblast, and acquire a naïve-like state of pluripotency, which is pivotal to differentiate PSCs into primordial germ cells (PGCs). Naïve culturing conditions induced a similar expression profile of pluripotency related genes in NWR iPSCs and human ESCs. Furthermore, naïve-like NWR iPSCs displayed increased expression of naïve and PGC marker genes, and a higher integration propensity into developing mouse embryos. As the conversion process was aided by ectopic BCL2 expression, and we observed integration of reprogramming factors, the NWR iPSCs presented here are unsuitable for gamete production. However, the gained insights into the developmental potential of both primed and naïve-like NWR iPSCs are fundamental for in future PGC-specification in order to rescue the species from extinction using cryopreserved somatic cells.

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Language(s): eng - English
 Dates: 2022-02-092022-03-08
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/s41598-022-07059-w
PMID: 35260583
 Degree: -

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Title: Scientific Reports
  Abbreviation : Sci. Rep.
Source Genre: Journal
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Publ. Info: London, UK : Nature Publishing Group
Pages: - Volume / Issue: 12 Sequence Number: 3100 Start / End Page: - Identifier: ISSN: 2045-2322
CoNE: https://pure.mpg.de/cone/journals/resource/2045-2322