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  Dynamical component exchange in a model phase separating system: an NMR-based approach

Pantoja, C. F., Zweckstetter, M., & Rezaei-Ghaleh, N. (2022). Dynamical component exchange in a model phase separating system: an NMR-based approach. PhysChemChemPhys, 24(10), 6169-6175. doi:10.1039/D2CP00042C.

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Pantoja, C. F., Author
Zweckstetter, M.1, Author              
Rezaei-Ghaleh, N.1, Author              
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1Research Group of Protein Structure Determination using NMR, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350128              

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 Abstract: Biomolecular phase separation plays a key role in the spatial organization of cellular activities. Dynamic formation and rapid component exchange between phase separated cellular bodies and their environment are crucial for their function. Here, we employ a well-established phase separating model system, namely, a triethylamine (TEA)–water mixture, and develop an NMR approach to detect the exchange of scaffolding TEA molecules between separate phases and determine the underlying exchange rate. We further demonstrate how the advantageous NMR properties of fluorine nuclei provide access to otherwise inaccessible exchange processes of a client molecule. The developed NMR-based approach allows quantitative monitoring of the effect of regulatory factors on component exchange and facilitates “exchange”-based screening and optimization of small molecules against druggable biomolecular targets located inside condensed phases.

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Language(s): eng - English
 Dates: 2022-02-162022-03-14
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1039/D2CP00042C
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Title: PhysChemChemPhys
Source Genre: Journal
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Pages: - Volume / Issue: 24 (10) Sequence Number: - Start / End Page: 6169 - 6175 Identifier: -