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  Engineering the Reductive Glycine Pathway: A Promising Synthetic Metabolism Approach for C1-Assimilation

Claassens, N. J., Satanowski, A., Bysani, V., Dronsella, B., Orsi, E., Rainaldi, V., et al. (2022). Engineering the Reductive Glycine Pathway: A Promising Synthetic Metabolism Approach for C1-Assimilation. In T. Scheper, & R. Ulber (Eds.), Advances in Biochemical Engineering/Biotechnology (pp. 299-350).

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 Creators:
Claassens, N. J.1, Author              
Satanowski, A.1, Author              
Bysani, V.R.1, Author              
Dronsella, B.1, Author              
Orsi, E.1, Author              
Rainaldi, V.1, Author              
Yilmaz, S.1, Author              
Wenk, S.1, Author              
Lindner, S. N.1, Author              
Affiliations:
1Systems and Synthetic Metabolism, Max Planck Research Groups, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_2035297              

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 Abstract: In recent years the reductive glycine pathway (rGlyP) has emerged as a promising pathway for the assimilation of formate and other sustainable C1-feedstocks for future biotechnology. It was originally proposed as an attractive “synthetic pathway” to support formatotrophic growth due to its high ATP efficiency, linear structure, and limited overlap with native pathways in most microbial hosts. Here, we present the current state of research on this pathway including breakthroughs on its engineering. Different variants of the rGlyP are discussed, including its core module for formate to glycine conversion, as well as varying modules for substrate conversion to formate, and glycine assimilation routes. Very recently, the rGlyP has been successfully implemented for synthetic formatotrophic growth, as well as for growth on methanol, in some bacterial hosts. We discuss the engineering strategies employed in these studies, including growth-coupled selection of functional pathway modules. We also compare the rGlyP to other natural and synthetic C1-assimilation pathways. Finally, we provide an outlook on open challenges and opportunities for the rGlyP, including its engineering into more biotechnological hosts, as well as the still-to-be realized production of value-added chemicals via this pathway. We expect that further research on the rGlyP will support the efficient use of sustainable C1-substrates in bioproduction.

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Language(s): eng - English
 Dates: 2022-04-02
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/10_2021_181
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Title: Advances in Biochemical Engineering/Biotechnology
Source Genre: Book
 Creator(s):
Scheper, Thomas, Editor
Ulber, Roland, Editor
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: 180 Sequence Number: - Start / End Page: 299 - 350 Identifier: -