English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  pH-triggered assembly of endomembrane multicompartments in synthetic cells

Lussier, F., Schröter, M., Diercks, N. J., Jahnke, K., Weber, C., Frey, C., et al. (2021). pH-triggered assembly of endomembrane multicompartments in synthetic cells. ACS Synthetic Biology, 11, 366-382. doi:10.1021/acssynbio.1c00472.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
ACSSynthBiol_11_2022_366.pdf (Any fulltext), 11MB
 
File Permalink:
-
Name:
ACSSynthBiol_11_2022_366.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ACSSynthBiol_11_2022_366_Suppl1.pdf (Supplementary material), 2MB
 
File Permalink:
-
Name:
ACSSynthBiol_11_2022_366_Suppl1.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ACSSynthBiol_11_2022_366_Suppl2.avi (Supplementary material), 2MB
 
File Permalink:
-
Name:
ACSSynthBiol_11_2022_366_Suppl2.avi
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ACSSynthBiol_11_2022_366_Suppl3.avi (Supplementary material), 3MB
 
File Permalink:
-
Name:
ACSSynthBiol_11_2022_366_Suppl3.avi
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
ACSSynthBiol_11_2022_366_Suppl4.avi (Supplementary material), 755KB
 
File Permalink:
-
Name:
ACSSynthBiol_11_2022_366_Suppl4.avi
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/x-msvideo
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-

Creators

show
hide
 Creators:
Lussier, Félix1, Author              
Schröter, Martin1, Author              
Diercks, Nicolas J.1, Author              
Jahnke, Kevin1, Author              
Weber, Cornelia1, Author              
Frey, Christoph1, Author              
Platzman, Ilia1, Author              
Spatz, Joachim P.1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: synthetic biology, self-assembly, giant unilamellar vesicle, multicompartments, droplet-based microfluidics, water-in-oil emulsion
 Abstract: By using electrostatic interactions as driving force to assemble vesicles, the droplet-stabilized method was recently applied to reconstitute and encapsulate proteins, or compartments, inside giant unilamellar vesicles (GUVs) to act as minimal synthetic cells. However, the droplet-stabilized approach exhibits low production efficiency associated with the troublesome release of the GUVs from the stabilized droplets, corresponding to a major hurdle for the droplet-stabilized approach. Herein, we report the use of pH as a potential trigger to self-assemble droplet-stabilized GUVs (dsGUVs) by either bulk or droplet-based microfluidics. Moreover, pH enables the generation of compartmentalized GUVs with flexibility and robustness. By co-encapsulating pH-sensitive small unilamellar vesicles (SUVs), negatively charged SUVs, and/or proteins, we show that acidification of the droplets efficiently produces dsGUVs while sequestrating the co-encapsulated material. Most importantly, the pH-mediated assembly of dsGUVs significantly improves the production efficiency of free-standing GUVs (i.e., released from the stabilizing-droplets) compared to its previous implementation.

Details

show
hide
Language(s): eng - English
 Dates: 2021-09-222021-12-10
 Publication Status: Published in print
 Pages: 17
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ACS Synthetic Biology
  Abbreviation : ACS Synth. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 11 Sequence Number: - Start / End Page: 366 - 382 Identifier: ISSN: 2161-5063
CoNE: https://pure.mpg.de/cone/journals/resource/2161-5063