English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Printing and erasing of DNA-based photoresists inside synthetic cells

Walther, T., Jahnke, K., Abele, T., & Göpfrich, K. (2022). Printing and erasing of DNA-based photoresists inside synthetic cells. Advanced Functional Materials, 32(25): 2200762, pp. 1-9. doi:10.1002/adfm.202200762.

Item is

Basic

show hide
Genre: Journal Article

Files

show Files
hide Files
:
AdvFunctMater_32_2022_2200762.pdf (Any fulltext), 6MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2022_2200762_Suppl1.pdf (Supplementary material), 2MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762_Suppl1.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2022_2200762_Suppl2.mp4 (Supplementary material), 5MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762_Suppl2.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2022_2200762_Suppl3.mp4 (Supplementary material), 4MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762_Suppl3.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2022_2200762_Suppl4.mp4 (Supplementary material), 8MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762_Suppl4.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2022_2200762_Suppl5.mp4 (Supplementary material), 5MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762_Suppl5.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2022_2200762_Suppl6.mp4 (Supplementary material), 5MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762_Suppl6.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2022_2200762_Suppl7.mp4 (Supplementary material), 5MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762_Suppl7.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
AdvFunctMater_32_2022_2200762_Suppl8.mp4 (Supplementary material), 4MB
 
File Permalink:
-
Name:
AdvFunctMater_32_2022_2200762_Suppl8.mp4
Description:
-
Visibility:
Restricted (Max Planck Institute for Medical Research, MHMF; )
MIME-Type / Checksum:
video/mp4
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-
Description:
-
Description:
-
Description:
-
Description:
-
Description:
-
Description:
-
Description:
-
Description:
-
Description:
-

Creators

show
hide
 Creators:
Walther, Tobias1, Author              
Jahnke, Kevin1, Author              
Abele, Tobias1, Author              
Göpfrich, Kerstin1, Author              
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

Content

show
hide
Free keywords: -
 Abstract: In the pursuit of producing functioning synthetic cells from the bottom-up, DNA nanotechnology has proven to be a powerful tool. However, the crowded yet highly organized arrangement in living cells, bridging from the nano- to the micron-scale, remains challenging to recreate with DNA-based architectures. Here, laser microprinting is established to print and erase shape-controlled DNA hydrogels inside the confinement of water-in-oil droplets and giant unilamellar lipid vesicles (GUVs). The DNA-based photoresist consists of a photocleavable inactive DNA linker, which interconnects Y-shaped DNA motifs when activated by local irradiation with a 405 nm laser. An alternative linker design allows to erase custom features from a preformed DNA hydrogel with feature sizes down to 1.38 μm. The present work demonstrates that the DNA hydrogels can serve as an internal support to stabilize non-spherical GUV shapes. Overall, DNA-based photoresists for laser printing in confinement allow to build up architectures on the interior of synthetic cells with light, which diversifies the toolbox of bottom-up synthetic biology.

Details

show
hide
Language(s): eng - English
 Dates: 2022-03-182022-06-17
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Advanced Functional Materials
  Abbreviation : Adv. Funct. Mater.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Weinheim : Wiley-VCH Verlag GmbH
Pages: - Volume / Issue: 32 (25) Sequence Number: 2200762 Start / End Page: 1 - 9 Identifier: ISSN: 1616-301X
CoNE: https://pure.mpg.de/cone/journals/resource/954925596563