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  Printing and erasing of DNA-based photoresists inside synthetic cells

Walther, T., Jahnke, K., Abele, T., & Göpfrich, K. (2022). Printing and erasing of DNA-based photoresists inside synthetic cells. Advanced Functional Materials, 32(25): 2200762, pp. 1-9. doi:10.1002/adfm.202200762.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-36E5-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000A-A576-7
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 Creators:
Walther, Tobias1, Author           
Jahnke, Kevin1, Author           
Abele, Tobias1, Author           
Göpfrich, Kerstin1, Author           
Affiliations:
1Cellular Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_2364731              

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 Abstract: In the pursuit of producing functioning synthetic cells from the bottom-up, DNA nanotechnology has proven to be a powerful tool. However, the crowded yet highly organized arrangement in living cells, bridging from the nano- to the micron-scale, remains challenging to recreate with DNA-based architectures. Here, laser microprinting is established to print and erase shape-controlled DNA hydrogels inside the confinement of water-in-oil droplets and giant unilamellar lipid vesicles (GUVs). The DNA-based photoresist consists of a photocleavable inactive DNA linker, which interconnects Y-shaped DNA motifs when activated by local irradiation with a 405 nm laser. An alternative linker design allows to erase custom features from a preformed DNA hydrogel with feature sizes down to 1.38 μm. The present work demonstrates that the DNA hydrogels can serve as an internal support to stabilize non-spherical GUV shapes. Overall, DNA-based photoresists for laser printing in confinement allow to build up architectures on the interior of synthetic cells with light, which diversifies the toolbox of bottom-up synthetic biology.

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Language(s): eng - English
 Dates: 2022-03-182022-06-17
 Publication Status: Published online
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/adfm.202200762
URI: https://www.webofscience.com/wos/woscc/full-record/WOS:000770370500001
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Title: Advanced Functional Materials
  Abbreviation : Adv. Funct. Mater.
Source Genre: Journal
 Creator(s):
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Publ. Info: Weinheim : Wiley-VCH Verlag GmbH
Pages: - Volume / Issue: 32 (25) Sequence Number: 2200762 Start / End Page: 1 - 9 Identifier: ISSN: 1616-301X
CoNE: https://pure.mpg.de/cone/journals/resource/954925596563