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  Mechanism of spindle pole organization and instability in human oocytes

So, C., Menelaou, K., Uraji, J., Harasimov, K., Steyer, A. M., Seres, K. B., et al. (2022). Mechanism of spindle pole organization and instability in human oocytes. Science, 375(6581): eabj3944. doi:10.1126/science.abj3944.

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 Creators:
So, C.1, Author           
Menelaou, K.1, Author           
Uraji, J.1, Author           
Harasimov, K.1, Author           
Steyer, A. M.2, Author           
Seres, K. B., Author
Bucevičius, J.3, Author
Lukinavicius, G.3, Author
Möbius, W.2, Author           
Sibold, C., Author
Tandler-Schneider, A., Author
Eckel, H., Author
Moltrecht, R., Author
Blayney, M., Author
Elder, K., Author
Schuh, M.1, Author           
Affiliations:
1Department of Meiosis, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350271              
2Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350301              
3Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_persistent22              

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 Abstract: Human oocytes are prone to assembling meiotic spindles with unstable poles, which can favor aneuploidy in human eggs. The underlying causes of spindle instability are unknown. We found that NUMA (nuclear mitotic apparatus protein)–mediated clustering of microtubule minus ends focused the spindle poles in human, bovine, and porcine oocytes and in mouse oocytes depleted of acentriolar microtubule-organizing centers (aMTOCs). However, unlike human oocytes, bovine, porcine, and aMTOC-free mouse oocytes have stable spindles. We identified the molecular motor KIFC1 (kinesin superfamily protein C1) as a spindle-stabilizing protein that is deficient in human oocytes. Depletion of KIFC1 recapitulated spindle instability in bovine and aMTOC-free mouse oocytes, and the introduction of exogenous KIFC1 rescued spindle instability in human oocytes. Thus, the deficiency of KIFC1 contributes to spindle instability in human oocytes.

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Language(s): eng - English
 Dates: 2022-022022-02-11
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1126/science.abj3944
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Title: Science
  Abbreviation : Science
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Association for the Advancement of Science
Pages: 19 Volume / Issue: 375 (6581) Sequence Number: eabj3944 Start / End Page: - Identifier: ISSN: 0036-8075
CoNE: https://pure.mpg.de/cone/journals/resource/991042748276600_1