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  A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks

Funk, J., Merino, F., Schaks, M., Rottner, K., Raunser, S., & Bieling, P. (2021). A barbed end interference mechanism reveals how capping protein promotes nucleation in branched actin networks. Nature Communications, 12(1): 5329. doi:10.1038/s41467-021-25682-5.

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 Creators:
Funk, J, Author
Merino, F1, 2, Author           
Schaks, M, Author
Rottner, K, Author
Raunser, S, Author
Bieling, P, Author
Affiliations:
1Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375791              
2Cytoskeletal Structure and Evolution Group, Department Protein Evolution, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3477693              

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 Abstract: Heterodimeric capping protein (CP/CapZ) is an essential factor for the assembly of branched actin networks, which push against cellular membranes to drive a large variety of cellular processes. Aside from terminating filament growth, CP potentiates the nucleation of actin filaments by the Arp2/3 complex in branched actin networks through an unclear mechanism. Here, we combine structural biology with in vitro reconstitution to demonstrate that CP not only terminates filament elongation, but indirectly stimulates the activity of Arp2/3 activating nucleation promoting factors (NPFs) by preventing their association to filament barbed ends. Key to this function is one of CP's C-terminal "tentacle" extensions, which sterically masks the main interaction site of the terminal actin protomer. Deletion of the β tentacle only modestly impairs capping. However, in the context of a growing branched actin network, its removal potently inhibits nucleation promoting factors by tethering them to capped filament ends. End tethering of NPFs prevents their loading with actin monomers required for activation of the Arp2/3 complex and thus strongly inhibits branched network assembly both in cells and reconstituted motility assays. Our results mechanistically explain how CP couples two opposed processes-capping and nucleation-in branched actin network assembly.

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Language(s): eng - English
 Dates: 2021-09
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-021-25682-5
PMID: 34504078
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: 17 Volume / Issue: 12 (1) Sequence Number: 5329 Start / End Page: - Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723