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  Strain and rupture of HIV-1 capsids during uncoating

Yu, A., Lee, E. M. Y., Briggs, J. A. G., Ganser-Pornillos, B. K., Pornillos, O., & Voth, G. A. (2022). Strain and rupture of HIV-1 capsids during uncoating. Proceedings of the National Academy of Sciences of the United States of America, 119(10): e2117781119. doi:10.1073/pnas.2117781119.

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 Creators:
Yu, Alvin1, Author
Lee, Elizabeth M. Y.1, Author
Briggs, John A. G.2, Author           
Ganser-Pornillos, Barbie K.1, Author
Pornillos, Owen1, Author
Voth, Gregory A.1, Author
Affiliations:
1external, ou_persistent22              
2Briggs, John / Cell and Virus Structure, Max Planck Institute of Biochemistry, Max Planck Society, ou_3344661              

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Free keywords: MOLECULAR-DYNAMICSScience & Technology - Other Topics; HIV capsid; all-atom molecular dynamics;   uncoating; strain;
 Abstract: Viral replication in HIV-1 relies on a fullerene-shaped capsid to transport genetic material deep into the nucleus of an infected cell. Capsid stability is linked to the presence of cofactors, including inositol hexakisphosphates (IP6) that bind to pores found in the capsid. Using extensive all-atom molecular dynamics simulations of HIV-1 cores imaged from cryo-electron tomography (cryoET) in intact virions, which contain IP6 and a ribonucleoprotein complex, we find markedly striated patterns of strain on capsid lattices. The presence of these cofactors also increases rigidity of the capsid. Conformational analysis of capsid proteins (CA) show CA accommodates strain by locally flexing away from structures resolved using X-ray crystallography and cryo-ET. Then, cryo-ET of HIV-1 cores undergoing endogenous reverse transcription demonstrates that lattice strain increases in the capsid prior to mechanical failure and that the capsid ruptures by crack propagation along regions of high strain. These results uncover HIV-1 capsid properties involved in their critical disassembly process.

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Language(s): eng - English
 Dates: 2022-03
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: ISI: 000772220900001
DOI: 10.1073/pnas.2117781119
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 119 (10) Sequence Number: e2117781119 Start / End Page: - Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230