Live-cell imaging of RNA transport in axons of cultured primary neurons

Hees, Jara Tabitha; Harbauer, Angelika B.

doi:10.1007/978-1-0716-1990-2_11

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Live-cell imaging of RNA transport in axons of cultured primary neurons

Hees, J. T., & Harbauer, A. B. (2022). Live-cell imaging of RNA transport in axons of cultured primary neurons. In A. Vagnoni (Ed.), Axonal transport. Methods and protocols (1, pp. 225-237). New York: Humana.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-533A-8 Version Permalink: https://hdl.handle.net/21.11116/0000-000D-2BC4-6

Genre: Book Chapter

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Creators:
Hees, Jara Tabitha^{1, 2, 3}, Author
Harbauer, Angelika B.², Author

Affiliations:
1IMPRS-BI: Martinsried, MPI of Neurobiology, Max Planck Society, ou_3510604
2Max Planck Research Group: Neurometabolism / Harbauer, MPI of Neurobiology, Max Planck Society, ou_3232739
3IMPRS-LS: Martinsried, MPI of Neurobiology, Max Planck Society, ou_3477841

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Free keywords: RNA transport MS2 PP7 Split-Venus Transient transfection Cultured primary neurons Imaging

Abstract: The use of fluorescent proteins has revolutionized the study of protein localization and transport. However, the visualization of other molecules and specifically RNA during live-cell imaging remains challenging. In this chapter, we provide guidance to the available methods, their advantages and drawbacks as well as provide a detailed protocol for the detection of RNA transport using the MS2/PP7-split-Venus system for background-free RNA imaging.

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Dates: Date issued: 2022-04-13

Publication Status: Issued

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Identifiers: Other: MEDLINE:35412279
DOI: 10.1007/978-1-0716-1990-2_11

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Title: Axonal transport. Methods and protocols

Source Genre: Book

Creator(s):
Vagnoni, Alessio, Editor

Affiliations:
-

Publ. Info: New York : Humana, 1

Pages: 573 Volume / Issue: - Sequence Number: - Start / End Page: 225 - 237 Identifier: ISBN: 978-1-0716-1989-6

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Title: Methods in molecular biology

Source Genre: Series

Creator(s):
Clifton, N. J., Editor

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-

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Pages: - Volume / Issue: 2431 Sequence Number: - Start / End Page: - Identifier: -