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  RuvB-like ATPases function in chromatin decondensation at the end of mitosis

Magalska, A., Schellhaus, A., Moreno Andrés, D., Zanini, F., Schooley, A., Sachdev, R., et al. (2014). RuvB-like ATPases function in chromatin decondensation at the end of mitosis. Developmental Cell, 31(3), 305-318. doi:10.1016/j.devcel.2014.09.001.

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 Creators:
Magalska, A, Author           
Schellhaus, AK, Author           
Moreno Andrés, D, Author           
Zanini, F1, Author           
Schooley, A, Author           
Sachdev, R, Author           
Schwarz, H2, Author           
Madlung, J, Author
Antonin, W, Author           
Affiliations:
1Research Group Evolutionary Dynamics and Biophysics, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3377926              
2Electron Microscopy, Max Planck Institute for Developmental Biology, Max Planck Society, Max-Planck-Ring 5, 72076 Tübingen, DE, ou_3375794              

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 Abstract: Chromatin undergoes extensive structural changes during the cell cycle. Upon mitotic entry, metazoan chromatin undergoes tremendous condensation, creating mitotic chromosomes with 50-fold greater compaction relative to interphase chromosomes. At the end of mitosis, chromosomes reestablish functional interphase chromatin competent for replication and transcription through a decondensation process that is cytologically well described. However, the underlying molecular events and factors remain unidentified. We describe a cell-free system that recapitulates chromatin decondensation based on purified mitotic chromatin and Xenopus egg extracts. Using biochemical fractionation, we identify RuvB-like ATPases as chromatin decondensation factors and demonstrate that their ATPase activity is essential for decondensation. Our results show that decompaction of metaphase chromosomes is not merely an inactivation of known chromatin condensation factors but rather an active process requiring specific molecular machinery. Our cell-free system provides an important tool for further molecular characterization of chromatin decondensation and its coordination with concomitant processes.

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 Dates: 2014-11
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: DOI: 10.1016/j.devcel.2014.09.001
PMID: 25443297
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Title: Developmental Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 31 (3) Sequence Number: - Start / End Page: 305 - 318 Identifier: ISSN: 1534-5807
CoNE: https://pure.mpg.de/cone/journals/resource/111006902714134