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  Automated laser-transfer synthesis of high-density microarrays for infectious disease screening

Paris, G., Heidepriem, J., Tsouka, A., Liu, Y., Mattes, D. S., Pinzón Martín, S., et al. (2022). Automated laser-transfer synthesis of high-density microarrays for infectious disease screening. Advanced Materials, 34(12): 2200359. doi:10.1002/adma.202200359.

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 Creators:
Paris, Grigori1, Author              
Heidepriem, Jasmin1, Author              
Tsouka, Alexandra1, Author              
Liu, Yuxin1, Author              
Mattes, Daniela S., Author
Pinzón Martín, Sandra2, Author              
Dallabernardina, Pietro1, Author              
Mende, Marco1, Author              
Lindner, Celina, Author
Wawrzinek, Robert3, Author              
Rademacher, Christoph, Author
Seeberger, Peter H.4, Author              
Breitling, Frank, Author
Bischoff, F. Ralf, Author
Wolf, Timo, Author
Löffler, Felix F.1, Author              
Affiliations:
1Felix Löffler, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2385692              
2Daniel Varón Silva, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863302              
3Christoph Rademacher, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863300              
4Peter H. Seeberger - Automated Systems, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863306              

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Free keywords: high-throughput; laser-induced forward transfer; peptides; Schiff base fluorophores; solid phase synthesis
 Abstract: Abstract Laser-induced forward transfer (LIFT) is a rapid laser-patterning technique for high-throughput combinatorial synthesis directly on glass slides. A lack of automation and precision limited LIFT applications to simple proof-of-concept syntheses of fewer than 100 compounds. Here, we report an automated synthesis instrument that combines laser transfer and robotics for parallel synthesis in a microarray format with up to 10000 individual reactions/cm2. An optimized pipeline for amide bond formation is the basis for preparing complex peptide microarrays with thousands of different sequences in high yield with high reproducibility. The resulting peptide arrays are of higher quality than commercial peptide arrays. More than 4800 15-residue peptides resembling the entire Ebola virus proteome on a microarray were synthesized to study the antibody response of an Ebola virus infection survivor. We identified known and unknown epitopes that serve now as a basis for Ebola diagnostic development. The versatility and precision of the synthesizer is demonstrated by in situ synthesis of fluorescent molecules via Schiff base reaction and multi-step patterning of precisely definable amounts of fluorophores. This automated laser transfer synthesis approach opens new avenues for high-throughput chemical synthesis and biological screening.

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Language(s): eng - English
 Dates: 2022-04-152022
 Publication Status: Published in print
 Pages: -
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 Rev. Type: -
 Identifiers: DOI: 10.1002/adma.202200359
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Title: Advanced Materials
  Abbreviation : Adv. Mater.
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH
Pages: - Volume / Issue: 34 (12) Sequence Number: 2200359 Start / End Page: - Identifier: ISSN: 0935-9648