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  A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy

Jiang, G., Ren, T.-B., D´Este, E., Xiong, M., Xiong, B., Johnsson, K., et al. (2022). A synergistic strategy to develop photostable and bright dyes with long Stokes shift for nanoscopy. Nature Communications, 13: 2264, pp. 1-10. doi:10.1038/s41467-022-29547-3.

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 Creators:
Jiang, Gangwei, Author
Ren, Tian-Bing, Author
D´Este, Elisa1, Author           
Xiong, Mengyi, Author
Xiong, Bin, Author
Johnsson, Kai2, Author           
Zhang, Xiao-Bing, Author
Wang, Lu, Author
Yuan, Lin, Author
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Chemical Biology, Max Planck Institute for Medical Research, Max Planck Society, ou_2364732              

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 Abstract: The quality and application of super-resolution fluorescence imaging greatly lie in the dyes’ properties, including photostability, brightness, and Stokes shift. Here we report a synergistic strategy to simultaneously improve such properties of regular fluorophores. Introduction of quinoxaline motif with fine-tuned electron density to conventional rhodamines generates new dyes with vibration structure and inhibited twisted-intramolecular-charge-transfer (TICT) formation synchronously, thus increasing the brightness and photostability while enlarging Stokes shift. The new fluorophore YL578 exhibits around twofold greater brightness and Stokes shift than its parental fluorophore, Rhodamine B. Importantly, in Stimulated Emission Depletion (STED) microscopy, YL578 derived probe possesses a superior photostability and thus renders threefold more frames than carbopyronine based probes (CPY-Halo and 580CP-Halo), known as photostable fluorophores for STED imaging. Furthermore, the strategy is well generalized to offer a new class of bright and photostable fluorescent probes with long Stokes shift (up to 136 nm) for bioimaging and biosensing.

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Language(s): eng - English
 Dates: 2021-09-082022-03-112022-07-04
 Publication Status: Published online
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/s41467-022-29547-3
 Degree: -

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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 13 Sequence Number: 2264 Start / End Page: 1 - 10 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723