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Abstract:
Commercialization ofBrassica napus.L (oilseed rape) meal as protein diet is gaining moreattention due to its well-balanced amino acid and protein contents. Phytic acid (PA) is a majorsource of phosphorus in plants but is considered as anti-nutritive for monogastric animalsincluding humans due to its adverse effects on essential mineral absorption. The undigested PAcauses eutrophication, which potentially threatens aquatic life. PA accounts to 2-5% in matureseeds of oilseed rape and is synthesized by complex pathways involving multiple enzymes.Breeding polyploids for recessive traits is challenging as gene functions are encoded by severalparalogs. Gene redundancy often requires to knock out several gene copies to study theirunderlying effects. Therefore, we adopted CRISPR-Cas9 mutagenesis to knock out threefunctional paralogs ofBnITPK. We obtained low PA mutants with an increase of free phosphorusin the canola grade spring cultivar Haydn. These mutants could mark an important milestone inrapeseed breeding with an increase in protein value and no adverse effects on oil contents.
