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  Regulation of MCSF receptors on microglia in the normal and injured mouse central nervous system: A quantitative immunofluorescence study using confocal laser microscopy

Raivich, G., Haas, S., Werner, A., Klein, M. A., Kloss, C. U. A., & Kreutzberg, G. W. (1998). Regulation of MCSF receptors on microglia in the normal and injured mouse central nervous system: A quantitative immunofluorescence study using confocal laser microscopy. The Journal of Comparative Neurology, 395(3), 342-358. doi:10.1002/(sici)1096-9861(19980808)395:3<342:Aid-cne6>3.0.Co;2-2.

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 Creators:
Raivich, G.1, Author           
Haas, Stefan1, Author           
Werner, Alexander1, Author           
Klein, Michael A.1, Author           
Kloss, Christian U. A.1, Author           
Kreutzberg, G. W.1, Author           
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1Emeritus Group: Neuromorphology / Kreutzberg, MPI of Neurobiology, Max Planck Society, ou_1113551              

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Free keywords: macrophage brain CSF-1 M-CSF autofluorescence colony-stimulating factor growth-factor-beta blood-brain-barrier rat facial nucleus immunohistochemical localization plasminogen-activator neurological disease alzheimers-disease messenger-rna factor gene Neurosciences & Neurology Zoology
 Abstract: The macrophage colony-stimulating factor (MCSF) is a 40-76-kD glycoprotein that plays an important role in the activation and proliferation of microglia both in vitro and in injured neural tissue. Here, we examined the regulation of MCSF receptor (MCSFR) and MCSF in the normal and injured mouse central nervous system (CNS) by using confocal laser microscopy, quantitative immunofluorescence, and reverse transcriptase-polymerase chain reaction (RT-PCR) techniques. Immunohistochemistry on fixed, Boating tissue sections demonstrated low to moderate MCSFR immunoreactivity (MCSFR-IR) on microglia in the gray and white matter throughout the mouse CNS in the forebrain, brainstem, cerebellum, and spinal cord. High levels of MCSFR-IR were restricted to the superficial layer of the spinal cord dorsal horn, substantia nigra, and area postrema, a CNS region that lacks the blood-brain barrier. CNS injury led to a strong and specific increase in MCSFR-IR in the directly injured dorsal forebrain, in the cervical spinal cord (C2) after transection of the sensory, minor occipital nerve, and in the axotomized facial motor nucleus. Further investigation at the mRNA level in the facial nucleus model showed that this increase was accompanied by a rapid induction of the transcript for MCSFR, with a peak 1-2 days after injury, but only a constitutive expression of MCSF-mRNA. In summary, although normal levels of MCSF receptor in most microglia are low microglial activation is accompanied by a rapid and massive increase. In view of the constitutive expression of MCSF, the early upregulation of the MCSF receptor may play a central role in preparing these macrophage-related cells to take part in the cellular response to CNS injury. (C) 1998 Wiley-Liss, Inc.

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Language(s): eng - English
 Dates: 1998
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: Other: WOS:000073401600006
DOI: 10.1002/(sici)1096-9861(19980808)395:3<342::Aid-cne6>3.0.Co;2-2
ISSN: 0021-9967
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Title: The Journal of Comparative Neurology
Source Genre: Journal
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Publ. Info: Hoboken, N.J. : John Wiley & Sons
Pages: - Volume / Issue: 395 (3) Sequence Number: - Start / End Page: 342 - 358 Identifier: ISSN: 1550-7130
CoNE: https://pure.mpg.de/cone/journals/resource/111088197763336