English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Sucrose synthases are not involved in starch synthesis in Arabidopsis leaves

Fünfgeld, M. F., Wang, W., Ishihara, H., Arrivault, S., Feil, R., Smith, A. M., et al. (2022). Sucrose synthases are not involved in starch synthesis in Arabidopsis leaves. Nature Plants, 8, 574-582. doi:10.1038/s41477-022-01140-y.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Fünfgeld, M. F.F.1, Author           
Wang, Wei2, Author
Ishihara, H.1, Author           
Arrivault, S.1, Author           
Feil, R.1, Author           
Smith, Alison M.2, Author
Stitt, M.1, Author           
Lunn, J. E.1, Author           
Niittylä, Totte2, Author
Affiliations:
1System Regulation, Department Stitt, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753327              
2external, ou_persistent22              

Content

show
hide
Free keywords: -
 Abstract: Many plants accumulate transitory starch reserves in their leaves during the day to buffer their carbohydrate supply against fluctuating light conditions, and to provide carbon and energy for survival at night. It is universally accepted that transitory starch is synthesized from ADP-glucose (ADPG) in the chloroplasts. However, the consensus that ADPG is made in the chloroplasts by ADPG pyrophosphorylase has been challenged by a controversial proposal that ADPG is made primarily in the cytosol, probably by sucrose synthase (SUS), and then imported into the chloroplasts. To resolve this long-standing controversy, we critically re-examined the experimental evidence that appears to conflict with the consensus pathway. We show that when precautions are taken to avoid artefactual changes during leaf sampling, Arabidopsis thaliana mutants that lack SUS activity in mesophyll cells (quadruple sus1234) or have no SUS activity (sextuple sus123456) have wild-type levels of ADPG and starch, while ADPG is 20 times lower in the pgm and adg1 mutants that are blocked in the consensus chloroplastic pathway of starch synthesis. We conclude that the ADPG needed for starch synthesis in leaves is synthesized primarily by ADPG pyrophosphorylase in the chloroplasts.

Details

show
hide
Language(s): eng - English
 Dates: 2022-04-282022-05
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1038/s41477-022-01140-y
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Plants
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Nature Publishing Group
Pages: - Volume / Issue: 8 Sequence Number: - Start / End Page: 574 - 582 Identifier: ISSN: 2055-0278
CoNE: https://pure.mpg.de/cone/journals/resource/2055-0278