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  Dual RNA-Seq of Chlamydia and Host Cells

Marsh, J., Hayward, R., Shetty, A., Mahurkar, A., Humphrys, M., & Myers, G. (2019). Dual RNA-Seq of Chlamydia and Host Cells. In Methods in Molecular Biology. US: Springer.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-673E-E Version Permalink: https://hdl.handle.net/21.11116/0000-000A-673F-D
Genre: Book Chapter

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 Creators:
Marsh, JW1, Author           
Hayward, RJ, Author
Shetty, A, Author
Mahurkar, A, Author
Humphrys, MS, Author
Myers, GSA, Author
Affiliations:
1Department Microbiome Science, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375789              

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 Abstract: During the infection of a host cell by a bacterial pathogen, a cascading series of gene expression changes occurs as each organism manipulates or responds to the other via defense or survival strategies. Unraveling this complex interplay is key for our understanding of bacterial virulence and host response pathways for the development of novel therapeutics. Dual RNA sequencing (dual RNA-Seq) has recently been developed to simultaneously capture host and bacterial transcriptomes from an infected cell. Leveraging the sensitivity and resolution allowed by RNA-seq, dual RNA-Seq can be applied to any bacteria-eukaryotic host interaction. We pioneered dual RNA-Seq to simultaneously capture Chlamydia and host expression profiles during an in vitro infection as proof of principle. Here we provide a detailed laboratory protocol and bioinformatics analysis guidelines for dual RNA-seq experiments focusing on Chlamydia as the organism of interest.

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 Dates: 2019
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-4939-9694-0_9
PMID: 31385273
 Degree: -

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Title: Methods in Molecular Biology
  Other : Methods Mol. Biol.
Source Genre: Series
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Publ. Info: US : Springer
Pages: - Volume / Issue: 2042 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745
CoNE: https://pure.mpg.de/cone/journals/resource/954927725544

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Title: Chlamydia trachomatis: Methods and Protocols
Source Genre: Book
 Creator(s):
Brown, AC, Editor
Affiliations:
-
Publ. Info: New York, NY, USA : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 123 - 135 Identifier: ISBN: 1-4939-9696-7