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  Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot

Deng, D., & Zi, Z. (2022). Absolute Quantification of TGF-β Signaling Proteins Using Quantitative Western Blot. In J. M. Walker (Ed.), Methods in Molecular Biology 2488: TGF-Beta Signaling (pp. 1-12). Totowa, New Jersey: Humana Press.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-68BE-C Version Permalink: https://hdl.handle.net/21.11116/0000-000A-F0AA-7
Genre: Book Chapter

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MethodsMolBiol_Deng and Zi_2022.pdf (Publisher version), 343KB
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 Creators:
Deng, Difan, Author
Zi, Zhike1, Author           
Affiliations:
1Cell Signaling Dynamics (Zhike Zi), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117284              

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Free keywords: Protein abundance; Quantitative immunoblotting; Smad3; Transforming Growth Factor-β
 Abstract: Cell signaling governs the basic functions of cells by molecular interactions that involve of many proteins. The abundance of signaling proteins can directly influence cellular responses to external signal, contributing to cellular heterogeneity. Absolute quantification of proteins is important for modeling and understanding the complex signaling network. Here, we introduce how to measure the amount of TGF-β signaling proteins using quantitative immunoblotting. In addition, we discuss how to convert the measurements of protein abundance to the quantities of absolute molecules per cell. This method is generally applicable to the absolute quantification of other proteins.

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Language(s): eng - English
 Dates: 2022
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-0716-2277-3_1
 Degree: -

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Title: Methods in Molecular Biology 2488: TGF-Beta Signaling
Source Genre: Series
 Creator(s):
Zi, Zhike1, Author           
Liu, Xuedong, Author
Walker, John M., Editor
Affiliations:
1 Cell Signaling Dynamics (Zhike Zi), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117284            
Publ. Info: Totowa, New Jersey : Humana Press
Pages: - Volume / Issue: 2488 Sequence Number: - Start / End Page: 1 - 12 Identifier: ISBN: 978-1-0716-2276-6 (print) 978-1-0716-2277-3 (online)
ISSN: 1064-3745 (print) 1940-6029 (online)