English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  ARR22 overexpression can suppress plant Two-Component Regulatory Systems

Wallmeroth, N., Jeschke, D., Slane, D., Nägele, J., Veerabagu, M., Mira-Rodado, V., et al. (2019). ARR22 overexpression can suppress plant Two-Component Regulatory Systems. PLoS One, 14(2): e0212056. doi:10.1371/journal.pone.0212056.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Wallmeroth, N, Author
Jeschke, D, Author
Slane, D1, Author           
Nägele, J, Author
Veerabagu, M, Author
Mira-Rodado, V, Author
Berendzen, KW, Author
Affiliations:
1Department Cell Biology, Max Planck Institute for Developmental Biology, Max Planck Society, ou_3375717              

Content

show
hide
Free keywords: -
 Abstract: In plants, several developmental processes are co-coordinated by cytokinins via phosphorylation dependent processes of the Two-Component System (TCS). An outstanding challenge is to track phosphorelay flow from cytokinin perception to its molecular outputs, of which gene activation plays a major role. To address this issue, a kinetic-based reporter system was expounded to track TCS phosphorelay activity in vivo that can distinguish between basal and cytokinin dependent effects of overexpressed TCS members. The TCS phosphorelay can be positively activated by cytokinin and inhibited by pharmaceuticals or naturally interfering components. In this case we took advantage of the phosphohistidine-phosphatase Arabidopsis Response Regulator (ARR) 22 and investigated its phosphocompetition with other TCS members in regulating promoters of ARR5 and WUS in Arabidopsis thaliana cell culture protoplasts. In congruency with the proposed function of ARR22, overexpression of ARR22 blocked the activation of all B-type ARRs in this study in a TCS dependent manner. Furthermore, this effect could not be mimicked by A-type response regulator overexpression or compensated by AHP overexpression. Compared to other reporter assays, ours mimicked effects previously observed only in transgenic plants for all of the TCS proteins studied, suggesting that it is possible to expose phosphocompetition. Thus, our approach can be used to investigate gene signaling networks involving the TCS by leveraging ARR22 as a TCS inhibitor along with B-type ARR overexpression.

Details

show
hide
Language(s):
 Dates: 2019-02
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1371/journal.pone.0212056
PMID: 30742656
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PLoS One
  Abbreviation : PLoS One
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: San Francisco, CA : Public Library of Science
Pages: 36 Volume / Issue: 14 (2) Sequence Number: e0212056 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: https://pure.mpg.de/cone/journals/resource/1000000000277850