English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Measurement of Flower Metabolite Concentrations Using Gas Chromatography–Mass Spectrometry and High-Performance Liquid Chromatography–Mass Spectrometry

Borghi, M., de Souza, L. P., & Fernie, A. R. (2022). Measurement of Flower Metabolite Concentrations Using Gas Chromatography–Mass Spectrometry and High-Performance Liquid Chromatography–Mass Spectrometry. In C. Lambing (Ed.), Methods in Molecular Biology. doi:10.1007/978-1-0716-2253-7_1.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Borghi, Monica1, Author
de Souza, L. P.2, Author              
Fernie, A. R.2, Author              
Affiliations:
1external, ou_persistent22              
2Central Metabolism, Department Willmitzer, Max Planck Institute of Molecular Plant Physiology, Max Planck Society, ou_1753339              

Content

show
hide
Free keywords: -
 Abstract: Metabolite profiling aiming at quantifying the metabolome of flowers is emerging as a suitable tool to understand the metabolic complexity of these reproductive organs and the associations between primary and secondary metabolites which characterize them. This chapter provides a general method for the combined analyses of primary and secondary metabolites via gas chromatography–mass spectrometry (GC-MS) and high-performance liquid chromatography–mass spectrometry (LC-MS) of flower samples. We describe the preparatory steps, the procedure of metabolites’ extraction and finally provide examples of data representation. The method described here can be applied to the analysis of metabolomes of entire flowers, as well as specific flower organs.

Details

show
hide
Language(s): eng - English
 Dates: 2022-04-222022
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-0716-2253-7_1
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Methods in Molecular Biology
Source Genre: Series
 Creator(s):
Lambing, C., Editor
Affiliations:
-
Publ. Info: -
Pages: - Volume / Issue: 2484 Sequence Number: - Start / End Page: - Identifier: -

Source 2

show
hide
Title: Plant Gametogenesis
Source Genre: Series
 Creator(s):
Affiliations:
Publ. Info: New York, NY : Humana
Pages: - Volume / Issue: 2484 Sequence Number: - Start / End Page: 3 - 12 Identifier: -