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  Reversibly switchable fluorescent Proteins for RESOLFT nanoscopy

Jensen, N. A., Jansen, I., Kamper, M., & Jakobs, S. (2020). Reversibly switchable fluorescent Proteins for RESOLFT nanoscopy. In T. Salditt, A. Egner, & D. R. Luke (Eds.), Nanoscale Photonic Imaging (pp. 241-261). Cham: Springer. doi:10.1007/978-3-030-34413-9_9.

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 Creators:
Jensen, N. A.1, Author              
Jansen, I.1, Author              
Kamper, M.1, Author              
Jakobs, S.2, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              

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 Abstract: Diffraction-limited lens-based optical microscopy fails to discern fluorescent features closer than ∼200 nm. All super-resolution microscopy (nanoscopy) approaches that fundamentally overcome the diffraction barrier rely on fluorophores that can adopt different states, typically a fluorescent ‘on-’state and a dark, non-fluorescent ‘off-’state. In reversible saturable optical linear fluorescence transitions (RESOLFT) nanoscopy, light is applied to induce transitions between two states and to switch fluorophores on and off at defined spatial coordinates. RESOLFT nanoscopy relies on metastable reversibly switchable fluorophores. Thereby, it is particularly suited for live-cell imaging, because it requires relatively low light levels to overcome the diffraction barrier. Most implementations of RESOLFT nanoscopy utilize reversibly photoswitchable fluorescent proteins (RSFPs), which are derivatives of proteins from the green fluorescent protein (GFP) family. In recent years, analysis of the molecular mechanisms of the switching processes have paved the way to a rational design of new RSFPs with superior characteristics for super-resolution microscopy. In this chapter, we focus on the newly developed RSFPs, the light-driven switching mechanisms and the use of RSFPs for RESOLFT nanoscopy.

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Language(s): eng - English
 Dates: 2020-06-102020
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1007/978-3-030-34413-9_9
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Title: Nanoscale Photonic Imaging
Source Genre: Book
 Creator(s):
Salditt, T., Editor
Egner, A., Editor
Luke, D. R., Editor
Affiliations:
-
Publ. Info: Cham : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 241 - 261 Identifier: ISBN: 978-3-030-34412-2
ISBN: 978-3-030-34413-9

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Title: Topics in Applied Physics
Source Genre: Series
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Pages: - Volume / Issue: 134 Sequence Number: - Start / End Page: - Identifier: -