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  Monitoring mitochondrial translation in living cells

Yousefi, R., Fornasiero, E. F., Cyganek, L., Montoya, J., Jakobs, S., Rizzoli, S. O., et al. (2021). Monitoring mitochondrial translation in living cells. EMBO Reports, 22(4): e51635. doi:10.15252/embr.202051635.

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 Creators:
Yousefi, R., Author
Fornasiero, E. F., Author
Cyganek, L., Author
Montoya, J., Author
Jakobs, S.1, Author              
Rizzoli, S. O., Author
Rehling, P.2, Author              
Pacheu-Grau, D., Author
Affiliations:
1Research Group of Mitochondrial Structure and Dynamics, MPI for biophysical chemistry, Max Planck Society, ou_578566              
2Max Planck Fellow Peter Rehling, ou_1298545              

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 Abstract: Mitochondria possess a small genome that codes for core subunits of the oxidative phosphorylation system and whose expression is essential for energy production. Information on the regulation and spatial organization of mitochondrial gene expression in the cellular context has been difficult to obtain. Here we devise an imaging approach to analyze mitochondrial translation within the context of single cells, by following the incorporation of clickable non-canonical amino acids. We apply this method to multiple cell types, including specialized cells such as cardiomyocytes and neurons, and monitor with spatial resolution mitochondrial translation in axons and dendrites. We also show that translation imaging allows to monitor mitochondrial protein expression in patient fibroblasts. Approaching mitochondrial translation with click chemistry opens new avenues to understand how mitochondrial biogenesis is integrated into the cellular context and can be used to assess mitochondrial gene expression in mitochondrial diseases.

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Language(s): eng - English
 Dates: 2021-02-152021-04-07
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.15252/embr.202051635
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Title: EMBO Reports
Source Genre: Journal
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Pages: 12 Volume / Issue: 22 (4) Sequence Number: e51635 Start / End Page: - Identifier: -