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  Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice

Gargareta, V.-I., Reuschenbach, J., Siems, S. B., Sun, T., Piepkorn, L., Mangana, C., et al. (2022). Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and mice. eLife, 11: e77019. doi:10.7554/eLife.77019.

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 Creators:
Gargareta, V.-I.1, Author              
Reuschenbach, J.1, Author              
Siems, S. B.1, Author              
Sun, T.1, Author              
Piepkorn, L.2, Author              
Mangana, C.1, Author              
Späte, E.1, Author              
Goebbels, S.1, Author              
Huitinga, I., Author
Möbius, W.1, Author              
Nave, K.-A.1, Author              
Jahn, O.2, Author              
Werner, Hauke B.1, Author              
Affiliations:
1Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350301              
2Department of Molecular Neurobiology, Max Planck Institute for Multidisciplinary Sciences, Max Planck Society, ou_3350300              

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Free keywords: Oligodendrocyte; axon-glia interaction; cross-species comparison; human; label-free proteomics; mouse; myelin sheath; neuroscience; scRNA-seq
 Abstract: Human myelin disorders are commonly studied in mouse models. Since both clades evolutionarily diverged approximately 85 million years ago, it is critical to know to what extent the myelin protein composition has remained similar. Here, we use quantitative proteomics to analyze myelin purified from human white matter and find that the relative abundance of the structural myelin proteins PLP, MBP, CNP, and SEPTIN8 correlates well with that in C57Bl/6N mice. Conversely, multiple other proteins were identified exclusively or predominantly in human or mouse myelin. This is exemplified by peripheral myelin protein 2 (PMP2), which was specific to human central nervous system myelin, while tetraspanin-2 (TSPAN2) and connexin-29 (CX29/ GJC3) were confined to mouse myelin. Assessing published scRNA-seq-datasets, human and mouse oligodendrocytes display well-correlating transcriptome profiles but divergent expression of distinct genes, including Pmp2, Tspan2, and Gjc3. A searchable web interface is accessible via www.mpinat.mpg.de/myelin. Species-dependent diversity of oligodendroglial mRNA expres- sion and myelin protein composition can be informative when translating from mouse models to humans.

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Language(s): eng - English
 Dates: 2022-05-11
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.7554/eLife.77019
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Title: eLife
Source Genre: Journal
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Pages: 25 Volume / Issue: 11 Sequence Number: e77019 Start / End Page: - Identifier: ISSN: 2050-084X