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  Optogenetic Control of TGF-β Signaling

Zi, Z., & Li, Y. (2022). Optogenetic Control of TGF-β Signaling. In Z. Zi, & Liu, Xuedong (Eds.), TGF-Beta Signaling (pp. 113-124). Totowa. New Jersey: Humana Press. doi:10.1007/978-1-0716-2277-3_9.

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Item Permalink: https://hdl.handle.net/21.11116/0000-000A-7315-D Version Permalink: https://hdl.handle.net/21.11116/0000-000A-739D-4
Genre: Book Chapter

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Li-Zi2022_Protocol_OptogeneticControlOfTGF-βSigna.pdf (Publisher version), 352KB
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 Creators:
Zi, Zhike1, Author           
Li, Yuchao1, Author           
Affiliations:
1Cell Signaling Dynamics (Zhike Zi), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117284              

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Free keywords: Optogenetics, Signaling transduction, TGF-β, Spatiotemporal precision, Live cell imaging
 Abstract: Cells employ signaling pathways to make decisions in response to changes in their immediate environment. The Transforming Growth Factor β (TGF-β) signaling pathway plays pivotal roles in regulating many cellular processes, including cell proliferation, differentiation, and migrations. In order to manipulate and explore the dynamic behavior of TGF-β signaling at high spatiotemporal resolution, we developed an optogenetic system (the optoTGFBRs system), in which light is used to control TGF-β signaling precisely in time and space. Here, we describe about experimental details of how to build the optoTGFBRs system and utilize it to manipulate TGF-β signaling in a single cell or a cell population using microscope or LED array, respectively.

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Language(s): eng - English
 Dates: 2022-03-29
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1007/978-1-0716-2277-3_9
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Title: TGF-Beta Signaling
Source Genre: Book
 Creator(s):
Zi, Zhike1, Editor           
Liu, Xuedong, Editor
Affiliations:
1 Cell Signaling Dynamics (Zhike Zi), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_2117284            
Publ. Info: Totowa. New Jersey : Humana Press
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 113 - 124 Identifier: ISBN: 978-1-0716-2276-6 (print) 978-1-0716-2277-3 (online)

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Title: Methods in Molecular Biology
Source Genre: Series
 Creator(s):
Walker, John M., Editor
Affiliations:
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Publ. Info: Totowa, New Jersey : Humana Press
Pages: - Volume / Issue: 2488 Sequence Number: - Start / End Page: - Identifier: ISSN: 1064-3745 (print) 1940-6029 (online)