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  Mitochondrial protein abundance gradients require the distribution of separated mitochondria

Bollmann, F., Dohrke, J.-N., Wurm, C. A., Jans, D. C., & Jakobs, S. (2021). Mitochondrial protein abundance gradients require the distribution of separated mitochondria. Biology, 10(7): 572. doi:10.3390/biology10070572.

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 Creators:
Bollmann, F.1, Author              
Dohrke, J.-N.2, Author              
Wurm, C. A.1, Author              
Jans, D. C.2, Author              
Jakobs, S.2, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              
2Research Group of Mitochondrial Structure and Dynamics, MPI for Biophysical Chemistry, Max Planck Society, ou_578566              

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Free keywords: image analysis; inner-cellular heterogeneity; nanoscopy; protein distribution; super-resolution microscopy
 Abstract: Mitochondria are highly dynamic organelles that interchange their contents mediated by fission and fusion. However, it has previously been shown that the mitochondria of cultured human epithelial cells exhibit a gradient in the relative abundance of several proteins, with the perinuclear mitochondria generally exhibiting a higher protein abundance than the peripheral mitochondria. The molecular mechanisms that are required for the establishment and the maintenance of such inner-cellular mitochondrial protein abundance gradients are unknown. We verified the existence of inner-cellular gradients in the abundance of clusters of the mitochondrial outer membrane protein Tom20 in the mitochondria of kidney epithelial cells from an African green monkey (Vero cells) using STED nanoscopy and confocal microscopy. We found that the Tom20 gradients are established immediately after cell division and require the presence of microtubules. Furthermore, the gradients are abrogated in hyperfused mitochondrial networks. Our results suggest that inner-cellular protein abundance gradients from the perinuclear to the peripheral mitochondria are established by the trafficking of individual mitochondria to their respective cellular destination.

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Language(s): eng - English
 Dates: 2021-06-23
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.3390/biology10070572
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Title: Biology
Source Genre: Journal
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Pages: 11 Volume / Issue: 10 (7) Sequence Number: 572 Start / End Page: - Identifier: -