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  Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish

Bensimon-Brito, A., Boezio, G. L. M., Cardeira-da-Silva, J., Wietelmann, A., Ramkumar, S., Lundegaard, P. R., et al. (2021). Integration of multiple imaging platforms to uncover cardiovascular defects in adult zebrafish. CARDIOVASCULAR RESEARCH, cvab310. doi:10.1093/cvr/cvab310.

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Bensimon-Brito, Anabela1, Author              
Boezio, Giulia L. M.1, Author              
Cardeira-da-Silva, Joao1, Author              
Wietelmann, Astrid2, Author              
Ramkumar, Srinath1, Author              
Lundegaard, Pia R., Author
Helker, Christian S. M.1, Author              
Ramadass, Radhan1, Author              
Piesker, Janett3, Author              
Nauerth, Arno, Author
Mueller, Clemens, Author
Stainier, Didier Y. R.1, Author              
Affiliations:
1Developmental Genetics, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591697              
2Small Animal Magnetic Resonance Imaging, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591708              
3Electron Microscopy, Max Planck Institute for Heart and Lung Research, Max Planck Society, ou_2591707              

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 Abstract: Aims Mammalian models have been instrumental in investigating adult heart function and human disease. However, electrophysiological differences with human hearts and high costs motivate the need for non-mammalian models. The zebrafish is a well-established genetic model to study cardiovascular development and function; however, analysis of cardiovascular phenotypes in adult specimens is particularly challenging as they are opaque. Methods and results Here, we optimized and combined multiple imaging techniques including echocardiography, magnetic resonance imaging, and micro-computed tomography to identify and analyse cardiovascular phenotypes in adult zebrafish. Using alk5a/tgfbr1a mutants as a case study, we observed morphological and functional cardiovascular defects that were undetected with conventional approaches. Correlation analysis of multiple parameters revealed an association between haemodynamic defects and structural alterations of the heart, as observed clinically. Conclusion We report a new, comprehensive, and sensitive platform to identify otherwise indiscernible cardiovascular phenotypes in adult zebrafish.

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 Dates: 2021-10-05
 Publication Status: Published online
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 Identifiers: ISI: 000789135100001
DOI: 10.1093/cvr/cvab310
PMID: 34609500
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Title: CARDIOVASCULAR RESEARCH
Source Genre: Journal
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Pages: - Volume / Issue: - Sequence Number: cvab310 Start / End Page: - Identifier: ISSN: 0008-6363